대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

탈명산(奪命散)이 배양심근세포(培養心筋細胞) 및 혈관평골근세포(血管平滑筋細胞)에 미치는 영향(影響)

Effects of Talmyung-san on the Cultured Rat Myocardiac Cell and Vascular Smooth Muscle Cell

  • 성강경 (원광대학교 광주한방병원) ;
  • 박세홍 (원광대학교 광주한방병원)
  • 발행 : 2000.08.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives : Talmyung-san(TMS) has been used for treatment of brain diseases in Chinese traditional medicine. However, little is known about the mechanism by which TMS rescues brain cells from ischemic damages. To elucidate the protective mechanisms of TMS, we execute experiments. Methods : The effects of TMS on ischemia/reperfusion-induced cytotoxicity and generation of nitric oxide(NO) are investigated in primary neonatal myocardial cells and A7rS, aortic smooth muscle cell line. Results : Ischemia/reperfusion itself induces severe myocardial cell death in vitro. However, treatment of the cells with TMS significantly reduces both ischemia/reperfusion-induced myocardial cell death and LDH release. In addition, pretreatment of TMS before reperfusion recovers the lose of beating rates alter ischemia/reperfusion. For a while, the water extract of TMS stimulates myocardial cells to produce NO in a dose dependent manner and it protects the damage of ischemia/reperfusion-induced myocardial cells. Furthermore, the protective effects of the water extract of TMS is mimicked by treatment of sodium nitroprusside, an exogenous NO donor. NG-monomethyl-L-arginine (NGMMA), a specific inhibitor of nitric oxide synthase(NOS), significantly blocks the protective effects of TMS on the cells after ischemia/reperfusion. In addition, on ischemia the water extract of TMS induce NO in A7r5 cell. Conclusions : Taken together, we suggest that the protective effects of TMS against ischemia/reperfusion-induced myocardial damages may be mediated by NO production of myocardial and vascular smooth muscle cell during ischemic condition.

키워드

참고문헌

  1. 大韓韓醫學會誌 v.18 no.1 三生飮이 實驗動物의 心血管系 및 血流障碍改善에 미치는 效果 全熙浚
  2. 笭桂朮甘湯 煎湯液이 心臟에 및 腎臟의 機能에 미치는 影響 金鍾和
  3. 心系內科學 全國韓醫科大學心系內科學敎室
  4. 中藥學 顔正華
  5. 臨床本草學 辛民敎
  6. HARRISON'S 내과학 해리슨 번역 편찬위원회
  7. 오늘의 진단 및 치료 강병철
  8. Color Atlas 임상의학 Forbes ${cdot}$ Jackson
  9. 심장학 서울대학교의과대학
  10. 家庭醫學 醫學敎育硏修院
  11. Katzung's 임상약리학 Katzung, Bertram G.
  12. 內科學(下) 李文鎬
  13. 申氏本草學 申佶求
  14. 臟腑辯證論治 金完熙;崔達永
  15. 生脈散이 實驗動物의 心筋細胞에 미치는 影響 申善澔
  16. 炙甘草湯이 培養心筋細胞에 미치는 影響 李來春
  17. 天南星의 修治方法에 따른 細胞毒性 硏究 朴炯培
  18. 半夏의 修治方法에 따른 細胞毒性 硏究 李秀培
  19. 허혈성 전처치의 심장보호 효과에 관한 연구:adenosine, protein kinase C 그리고 ATP 의존성 potassium channel의 역할 김대중
  20. 허혈성 전처치에 의한 심장보호 효과에 관한 연구 김현
  21. Nitric Oxide와 Peroxynitrite가 Murine Bladder Tumor-2 세포주의 고사에 미치는 영향 박현철
  22. 大韓韓醫學會誌 v.16 no.1 赤何首烏가 高Cholesterol食餌에 의하여 誘發된 家兎 冠狀動脈의 粥狀硬化에 미치는 影響 이원철
  23. 導痰湯이 高脂血症 實驗動物에 미치는 效果 孫成熙
  24. 四君子湯 二陳湯 및 六君子湯이 高脂血症에 미치는 影響 李承宰
  25. J. Mol. Cell Cardiol. v.5 Abrupt reoxygenation of the anoxic potassium-arrested perfused rat heart : a study of myocardial enzyme release Hearse, D.J.;Humphrey, S.M.;Chain, E.B.
  26. Heart Circ Physiol. v.15 Myocardial alteration due to free radical generation Burton, K.P.;McCord, J.M.;Ghai, G.
  27. Heart Cric. Physiol. v.29 Temporary contractile blockade pervents hypercontracture in anoxic-reoxygenated cardiomyocytes Siegmund, B.;Klietz, T.;Schwartz, P.;Piper, H.M.
  28. J. Mol. Cell Cardiol. v.10 Lysosomal alterations in ischemic myocardium : results or causes of myocardial damage Wildenthal, K.
  29. Am. J. Cardiol. v.44 Cellular calcium and cardiac cell death Katz, A.M.;Reuter, H.
  30. Am. J. Pathol. v.102 Lethal myocardial ischemic injury Jennings, R.B.;Reimer, K.A.
  31. Cir. Res. v.49 Total ischemia in dog hearts in vitro I. Comparision of high energy phosphate production, utilization, and depletion, and of adenine nucleotide catabolism in total ischemia in vitro VS severe ischemia in vivo Jennings, R.B.;Reimer, K.A.;Hill, M.L.;Mayer, S.E.
  32. Am. J. Pathol. v.102 The role of calcium in the ischemic myocardium Nayler, W.G.
  33. Hypertension v.20 Oxygen free radicals and cardiac reperfusion abnormalities Goldhaber, J.I.;Weiss, J.N.
  34. Circ. Res. v.81 Evidence that late preconditioning against myocadial stunning in conscious rabbits is triggered by the generation of nitric oxide Bolli, R.;Bhatti, Z.A.;Tang, X.L.;Qiu, Y.;Zhang, Q.;Guo, Y.(et al.)
  35. Circ. Res. v.83 Nitric oxide donors induce late preconditioning against mycardial stunning and infarction in conscious rabbits via an antioxidant-sensitive mechanism Takano, H.;Tang, X.L.;Qiu, Y.;Guo, Y.;French, B.A.;Bolli, R.
  36. Transplatation v.66 Protective effect of ischemic preconditioning on liver preservation-reperfusion injury in rats Yin, D.P.;Sankary, H.N.;Chong, A.S.;Ma, L.L.;Shen, J.;Foster, P.(et al.)
  37. Circulation v.98 Nitric oxide synthase is the mediator of late preconditioning ahasinst mycardial infraction inconscious rabbits Takano, H.;Manchikalapudi, S.;Tang, X.L.;Qiu, Y.;Rizvi, A.;Jadoon, A.K.(et al.)
  38. Cell v.80 NF-kB : alesson in family values Thanos, T.;Maniatis, T.
  39. J. Mol. Cell Cardiol. v.29 Role of inducible nitric oxide synthase in pharmacological preconditioning with monophosphoryl lipid A Zhao, L.;Weber, P.A.;Smith, J.R.;Comerford, M.L.;Elliott, G.T.
  40. Br. J. Pharmacol. v.113 Prevention by dexamethasone of the markedantiarrhythmic effects of preconditioning induced 20th after rapid cardiac pacing Vegh, A.;Papp, J.G.;Paratt, J.R.
  41. J. Immunol. v.150 Activation of human peripheral blood mononuclear cells by nitric oxide generating compounds Lander, H.M.;Sehajpal, P.;Levine, D.M.;Novogrodsky, A.
  42. J. Cardiovascular Surgery v.16 Ischemia - tolerance following cardioplegia arrest in human patients and in experimental animals Schaper, W.;Schaper, J.;Pulmowski, J.;Thiedmann, V.;Hehrlein, F.
  43. Br. J. Pharmacol. v.115 Involvement of protein kinase C in the delayer cyroprotection following sublethal ischemiae in rabbit myocardium Baxter, G.F.;Goma, F.M.;Yellon, D.M.
  44. Circulation v.98 Nitric oxide synthase is the mediator of late preconditioning against myocardial infarction in conscious rabbits Takano, H.;Manchikalapudi, S.;Tang, X.L.;Qiu, Y.;Rizvi, A.;Jadoon, A.K.(et al.)
  45. Basic Res. Cardiol. v.91 The early and late phases of preconditioning against myocardial stunning and the essential role of oxyradicals in the late phase an overview Bolli, R.
  46. Jpn. Circ. J. v.61 no.2 The role of nitric oxide in cardiac ischemia-reperfusion injury Nonami, Y.
  47. J. Immune. Meth. v.65 Rapid for celluar growth and survival, application to proliferation and cytotoxicity assays Mosman, T.