Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Protection by Sunghyangchungisan against Oxidative Endothelial Cell Injury

배양(培養)된 혈관(血管) 내피세포(內皮細胞)에서 산화성(酸化性) 세포(細胞) 손상(損傷)에 미치는 성향정기산(星香正氣散)의 보호(保護) 효과(效果)

  • Lee Dong-Uhn (Dept. of Oriental Medicine, Graduate School, Dong-Eui University) ;
  • Kim Young-Kyun (Dept. of Oriental Medicine, Graduate School, Dong-Eui University)
  • 이동언 (동의대학교 대학원 한의학과 내과학교실) ;
  • 김영균 (동의대학교 대학원 한의학과 내과학교실)
  • Published : 2000.12.05
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Abstract

Reactive oxygen species (ROS) play an important role in the pathogenesis of a variety of life threatening conditions such as atherosclerosis, myocardial infarction and cerebral stroke. In this study, the effect of Sunghyangchungisan (SHCS) as a cytoproctant against ROS-induced cell injury was studied by investigating its effect on $H_{2}O_2-induced$ cell injury in cultured endothelial cells derived from the human umbilical vein. SHCS effectively proteced the cells against $H_{2}O_2-induced$ injury determined by trypan blue exclusion ability and lactate dehydrogenase (LDH) release. The effect of SHCS was concentration-dependent and the concentrations to inhibit by 50% the cell death and LDH release were $0.9{\pm}0.1$ and $1.2{\pm}0.1\;mg/ml$, respectively. In addition, SHCS effectively protected the cells against t-butylhydroperoside- and menadione-Induced injury as well. SHCS inhibited lipid peroxidation determined by malondialdehyde production. SHCS exerted as an effective scavenger of ROS produced by exposing the cells to $H_{2}O_2$ The activities of the intracellular ROS scavenging enzymes such as superoxide dismutase, catalase and glutathione peroxidase were not Influenced by SHCS.These results indicate that SHCS might exert as an effective cytoprotectant against ROS-induced cell injury. Further intensive studies would provide us insights into mechanisms of the pharmacological actions of SHCS.

Keywords

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