Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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A STUDY ON A CULTURE OF HUMAN ALVEOLAR BONE CELLS

사람 치조골세포의 배양에 관한 연구

  • Choi, Byung-Ho (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Yonsei University) ;
  • Park, Jin-Hyung (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Yonsei University) ;
  • Yoo, Jae-Ha (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Yonsei University)
  • 최병호 (연세대학교 치과대학 구강악안면외과학교실 (원주기독병원)) ;
  • 박진형 (연세대학교 치과대학 구강악안면외과학교실 (원주기독병원)) ;
  • 유재하 (연세대학교 치과대학 구강악안면외과학교실 (원주기독병원))
  • Published : 2000.12.31
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Abstract

Human alveolar bone cells were isolated from alveolar bone fragments obtained from normal individual undergoing third molar extractions. Alveolar bone fragments were cultured as explant. Cells began to migrate in the first $5{\sim}7$ day and were confluent in $5{\sim}7$ week. Matrix mineralization was observed by 4 week. Our studies utilize established protocols for the characterization of these cells as osteoblasts by means of alkaline phosphatase activity determination, identification of osteocalcin antigens, establishing the presence of cells expressing type I collagen and determining the ability of cells to produce calcification. Transmission electron microscopic observations confirmed the presence of a collagen matrix undergoing a mineralization process. This new model, using human alveolar bone cells, may provide a tool to investigate alveolar bone development and physiology and to set up new therapeutic approaches.

Keywords