Journal of the Korean Association of Oral and Maxillofacial Surgeons
- 제26권1호
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- Pages.45-52
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- 2000
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- 2234-7550(pISSN)
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- 2234-5930(eISSN)
구강종양에서 p53 종양억제 유전자의 돌연변이에 관한 연구
A STUDY ON MUTATIONS OF P53 TUMOR SUPPRESSOR GENE IN ORAL TUMORS
- Joo, Seong-Chai (Phil Private Dental Clinic) ;
- Pyo, Sung-Woon (Dept. of Oral & Maxillofacial Surgery, Holy Family Hospital, The Catholic University)
- 발행 : 2000.02.28
초록
Nowadays, there are a lot of evidence that mutation of the p53 tumor suppressor gene is one of the most common genetic abnormalities in neoplastic progression. In this study, we analyzed 20 specimens of oral tumors(squamous cell carcinoma 14 cases, ameloblastoma 3 cases, adenoid cystic carcinoma 2 cases, malignant schwannoma 1 case)using polymerase chain reaction and direct sequencing which used an automated DNA sequencer and software for detection of mutations. Polymerase chain reactions were performed with 4 sets of primers encompassing exon 5, 6, 7, 8, and direct sequencing method was employed. The results were as followings. 1. We detected 10 point mutations out of 20 specimens (50%). 2. The genetic alterations included 7 mis-sense mutations resulting in single amino acid subtitutions, 2 silent mutations, 1 non-sense mutations encoding a stop codon. 3. Mutations were mostly in exon 7(7 out of 10 mutations, 70%) and involved codons 225, 234, 235, 236, 238, 247. 4. Therse were 4 cases of
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