Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Cytotoxicity and antimicrobial effects of the methanolic extract of Sophora flavescens Ait. (IV)

  • Baek, Seung-Hwa (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kang, Kil-Ung (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee, Jeong-Ho (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Park, Nang-Kyu (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Chai, Kyu-Yun (Division of Chemistry Technology and Biological Science, Wonkwang University) ;
  • You, Il-Soo (Department of Chemical Engineering, Iksan College) ;
  • Kim, Jong-Soo (Department of Chemical Engineering, Iksan College) ;
  • Ryu, Do-Gon (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Lee, Kang-Min (Department of Molecular Biology, Junbuk University) ;
  • Yang, Eun-Yeong (Kuhnil Pharmaceutical Co. LTD.) ;
  • Lee, Hyun-Ok (Department of Hygiene, Wonkwang Health College)
  • Published : 2000.08.30
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Abstract

This study was carried out to evaluate cytotoxicity of the methanol extract from Sophora flavescens Ait. against L1210 (lymphocytic leukemia) and $P388D_1$ (lymphoid neoplasma) Cells in vitro. We have determined cytotoxicity by the MTT (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H- tetrazolium bromide) assay. The order of cytotoxicity of Sophora flavescens Ait. extracts against L1210 and $P388D_1$ cells in vitro is as follows: Fr. 4 > Fr. 3 > Fr. 5 > Fr. 2 > Fr. 1. These results suggest that the fraction 4 of the methanol extracts from Sophora flavescens Ait. may be a valuable choice for the development of antitumor agents. In order to develop an antimicrobial agent, dried Sophora flavescens Ait. was extracted with hot methanol, and then antimicrobial activity (MIC test) was investigated. In this study, the fraction 3 of the methanol extracts from the roots of S. flavescens showed strong growth inhibition activity against gram-positive and gram-negative bacteria (MIC, $3.125\;{\mu}g/ml$) such as S. mutans, S. epidermidis and P. putida. These results indicate that fractions 3 and 4 inhibit tumor cells and bacteria.

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