Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Searching of Cyclin-Dependent Kinase 4/Cyclin D1 Enzyme Inhibition Materials from the Native Plants

자생 식물로 부터 Cyclin-dependent Kinase 4/Cyclin D1 저해물질의 탐색

  • Kim, Mi-Ran (Department of Agricultural Chemistry, Graduate School, Chungnam National University) ;
  • Ha, Ji-Hong (Department of Genetic engineering, Graduate School, Kyungpook National University) ;
  • Kwon, Byung-Mok (Protein Regulator RU, Korea Research Institute of Bioscience & Biotechnology) ;
  • Chung, Ha-Won (Protein Regulator RU, Korea Research Institute of Bioscience & Biotechnology) ;
  • Ahn, Byung-Tae (Protein Regulator RU, Korea Research Institute of Bioscience & Biotechnology) ;
  • Ryu, Shi-Yong (Korea Research Institute of Chemical Technology) ;
  • Sung, Nack-Do (Department of Agricultural Chemistry, Graduate School, Chungnam National University)
  • 김미란 (충남대학교 농과대학 응용생물화학부) ;
  • 하지홍 (경북대학교 자연과학대학 유전공학과) ;
  • 권병목 (생명공학연구소 항생물질연구실) ;
  • 정하원 (생명공학연구소 항생물질연구실) ;
  • 안병태 (생명공학연구소 항생물질연구실) ;
  • 유시용 (한국화학연구소 화학물질 연구부) ;
  • 성낙도 (충남대학교 농과대학 응용생물화학부)
  • Published : 2000.08.31
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Abstract

To search CDK4/Cyclin D1 enzyme inhibition materials, methanol extracts of native eighty seven plant species in thirty seven families were screened in vitro for their inhibiting activities against CDK4/Cyclin D1 enzyme which are control to the normal cell division cycle in human body. Extracts of Paeonia suffruticosa, Saurus chinensis, Sanguisorba officinalis and Celastrus orbiculatus among them significantly inhibited above fifty percent $(in\;5\;{\mu}g/ml)$ against CDK4/ Cyclin D1 enzyme. Especially, the extracts of P. suffruticosa and S. officinalis showed moderately strong inhibition. Also, cryptotanshinone was identified as active compound from a extracts of Salvia mitiorrhiza by spectroscopic analyses including 2D NMR experiments.

인체 세포의 세포주기를 조절하는 Cyclin-dependent Kinase(CDK) 중 CDK4/Cyclin D1 효소 저해 물질을 찾기 위하여 국내에 자생하는 37과 87종의 식물 추출물을 탐색한 결과, 50%$(5\;{\mu}g/ml)$ 이상의 CDK4/Cyclin D1 호소 저해활성을 보인 것은 단삼(Salvia miltiorrhiza), 노박덩굴(Celastrus orbiculatus), 삼백초(Saurus chinensis), 목단(Paeonia suffruticosa) 및 오이풀(Sanguisorba officinalis)이었다. 이들 중에서 목단과 오이풀 추출물이 가장 높은 56%의 저해율을 각각 나타내었다. 또한 추출물에서 활성물질을 추출 정제하여 2차원 NMR 등을 포함한 각종 기기분석을 통하여 cryptotanshinone으로 동정하였다.

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