Journal of Pharmaceutical Investigation
- 제30권4호
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- Pages.253-258
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- 2000
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- 2093-5552(pISSN)
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- 2093-6214(eISSN)
경구용 백신수송체용 GFP 함유 마이크로스피어의 제조 및 평가
Preparation and evaluation of GFP-containing microspheres for oral vaccine delivery system
- Jiang, Ge (College of Pharmacy, Chungnam National University) ;
- Park, Jong-Pil (College of Pharmacy, Chungnam National University) ;
- Kwak, Son-Hyok (College of Pharmacy, Chungnam National University) ;
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Hwang, Sung-Joo
(College of Pharmacy, Chungnam National University) ;
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Maeng, Pil-Jae
(Department of Microbiology, College of Natural Science, Chungnam National University)
- 발행 : 2000.12.20
초록
In order to design the oral vaccine delivery system, we prepared the alginate micro spheres containing GFP (green fluorescent protein) as a model drug by spray method. To optimize the preparation conditions of microspheres, we investigated the effects of various parameters including nozzle pressure, nozzle opening angle, and concentrations of sodium alginate and calcium chloride. The prepared microspheres were evaluated by measuring their sizes, loading efficiency, and morphology. The particle size of microspheres was affected by the concentration of sodium alginate and calcium chloride, nozzle pressure, and nozzle opening angle. As the concentration of sodium alginate increased, GFP loading efficiency and particles size of microsphere also increased. However, it was observed to be difficult to spray the sodium alginate solution with concentration greater than 1.5% (w/v), due to high viscosity. The pressure over