한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제32권3호
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- Pages.531-537
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- 2000
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- 0367-6293(pISSN)
DNA Comet Assay를 이용한 과일의 방사선 조사 확인
Detection of Irradiated Fruits Using the DNA Comet Assay
- Oh, Kyong-Nam (Detection laboratory Irradiated Foods, Korea Atomic Energy Research Institute) ;
- Park, Jun-Young (Detection laboratory Irradiated Foods, Korea Atomic Energy Research Institute) ;
- Kim, Kyeung-Eun (Detection laboratory Irradiated Foods, Korea Atomic Energy Research Institute) ;
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Yang, Jae-Seung
(Detection laboratory Irradiated Foods, Korea Atomic Energy Research Institute)
- 발행 : 2000.06.30
초록
과일의 방사선 조사 여부를 DNA comet assay로 확인하였다. 포도, 자두, 딸기, 복숭아, 사과, 천도복숭아를 구입하여 1.0kGy이하의 저선량으로 조사하고 비조사 시료와 조사시료간의 DNA 손상청도를 육안 검사 및 측정된 comet tail length로 비교하였다. 모든 시료에서 비조사 시료보다 조사시료의 tail length가 더 길었으며 포도와 자두는
The simple microgel electrophoresis of single cells, a 'comet assay', on fruit seeds enabled the rapid identification of irradiated fruits by comparing the intact non-irradiated cells and the damaged cells of irradiated fruits. Grapes and plums were irradiated with 0.1, 0.5, 0.7, 1.0 kGy and strawberries, peaches, apples, and nectarines were irradiated with only 1.0 kGy. Seeds were isolated, crushed, and the suspended cells were embedded in an agarose layer. After lysis of the cells, they were subjected to microgel electrophoresis for 2 minutes, and then stained. The DNA radiation-induced fragmentation of all the fruits stretched and migrated out of the cells forming a tail toward the anode giving the appearance of a comet, while the undamaged cells appeared as intact nuclei without tails. Grape and plum seeds irradiated at 0.5 kGy and higher showed significant increases in tail length. With increasing the irradiation doses, longer extention of the DNA from the nucleus toward the anode was observed. Strawberry, peach, apple, and nectarine seeds irradiated with 1.0 kGy also showed the longer tails than non-irradiated ones. DNA comet assay as a rapid and inexpensive screening technique could be an officially validated method for the detection of irradiated fruits.