Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 32 Issue 2
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- Pages.448-453
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- 2000
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- 0367-6293(pISSN)
An Apoptosis Regulator isolated from Petasites japonicum
머위로부터 분리한 apoptosis 조절물질
- Lee, Choong-Hwan (Enzyme Inhibition Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Chung, Myung-Chul (Enzyme Inhibition Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Lee, Ho-Jae (Enzyme Inhibition Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Kho, Yung-Hee (Enzyme Inhibition Research Unit, Korea Research Institute of Bioscience and Biotechnology)
- Published : 2000.04.30
Abstract
During the screening of inhibitors of caspase-3 induction in U937 human monocytic leukemia cells from natural sources, Petasites japonicum, which showed a high level of inhibition was selected. The inhibiting compound was purified from the methanol extract by Sephadex LH-20 column chromatography and HPLC. The inhibitor was identified as [3-(3,4-dihydroxyphenyl)-2-oxopropyl]ester, petasiphenol, by spectroscopic methods of UV, EIMS,
천연물로부터 U937 세포주를 사용한 caspase-3 유도저해물질을 탐색한 결과 머위(Petasites japonicum)를 선발하였다. 머위의 메탄올 추출물로부터 Sephadex LH-20 column chromatography, HPLC 등을 사용하여 저해물질을 분리하였으며, UV, EIMS, 1H-NMR,