Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Anti-tumor Promoting Activity of Some Malaysian Traditional Vegetable (Ulam) Extracts by Immunoblotting Analysis of Raji Cells

  • Ali, A.M. (Department of Biotechnology, Universiti Putra Malaysia) ;
  • Mooi, L.Y. (Department of Biotechnology, Universiti Putra Malaysia) ;
  • Yih, K. Yih (Department of Biotechnology, Universiti Putra Malaysia) ;
  • Norhanom, A.W. (Centre for the Foundation Studies in Science, University of Malaya) ;
  • Saleh, K. Mat (School of Bioscience and Biotechnology, Faculty of Sciene and Technology, Universiti Kebangsaan Malaysia) ;
  • Lajis, N.H. (Department of Chemistry, Universiti Putra Malaysia) ;
  • Yazid, A.M. (Department of Food Technology, Universiti Putra Malaysia) ;
  • Ahmad, F.B.H. (Department of Chemistry, Universiti Putra Malaysia) ;
  • Prasad, U. (University Hospital Kuala Lumpur, University of Malaya)
  • Published : 2000.09.01
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Abstract

The extracts of Carica papaya (flower), Barringtonia macrostachya (leaves), Coleus tuberosus (tuber), Mangifera indica (fruit skin) and Eugenia polyantha (leaves) showed strong in vitro anti-tumor promoting activity when assayed using Raji cells (Mooi et al., 1999). The antitumor promoting activity of the crude extracts was further analyzed by immunoblotting analysis of Raji cells carving Epstein-Barr virus genome. The expression of early antigens diffuse (EA-D) and early antigens restricted (EA-R) was determined by performing western blotting of treated Raji cells with human sera of nasopharyngeal carcinoma patients. All the plant extracts were shown to be able to suppress both EA-D and EA-R.

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