The Korean Journal of Physiology and Pharmacology
- Volume 4 Issue 1
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- Pages.63-72
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- 2000
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- 1226-4512(pISSN)
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- 2093-3827(eISSN)
Cadmium Inhibition of Renal Endosomal Acidification
- Kim, Moo-Seong (Department of Physiology, Kosin Medical College) ;
- Kim, Kyoung-Ryong (Department of Physiology, Kosin Medical College) ;
- Ahn, Do-Whan (Department of Physiology, Kosin Medical College) ;
- Park, Yang-Saeng (Department of Physiology, Kosin Medical College)
- Published : 2000.02.21
Abstract
Chronic exposure to cadmium (Cd) results in an inhibition of protein endocytosis in the renal proximal tubule, leading to proteinuria. In order to gain insight into the mechanism by which Cd impairs the protein endocytosis, we investigated the effect of Cd on the acidification of renal cortical endocytotic vesicles (endosomes). The endosomal acidification was assessed by measuring the pH gradient-dependent fluorescence change, using acridine orange or FITC-dextran as a probe. In renal endosomes isolated from Cd-intoxicated rats, the