약학회지 (YAKHAK HOEJI)
- 제44권6호
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- Pages.588-594
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- 2000
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
면역글로부린 G 생성에 대한 아이오타 - 카라기난의 어쥬번트 효과
Adjuvant Effects of Iota-Carrageenan on the Immunoglobulin G Production
- Koo, Bon-Woong (College of Pharmacy, Chung-Ang University) ;
- Lee, Kwang-Jae (College of Pharmacy, Chung-Ang University) ;
- Kim, Man-Suk (College of Pharmacy, Chung-Ang University) ;
- Kim, Ha-Hyung (College of Pharmacy, Chung-Ang University)
- 발행 : 2000.12.30
초록
To investigate the effects of iota-carrageenan (CAR) and/or alum on the adjuvancity as well as the structural difference of oligosaccharide on the IgG2b in the adjuvant effect, C57BL/6 mice were immunized twice with fetuin as a model antigen. CAR alone showed no significant effect on induction of antibody except IgG1. In contrast, Alum-CAR (after mixing of antigen-Alum, CAR adjuvant was prepared) and CAR-Alum (after formulation of antigen-CAR, Alum adjuvant was prepared) enhanced production of antibody, especially, IgG2b. After separation of IgG2b, changes of glycosylation were investigated using enzymelinked lectin assay. High affinity of IgG2b to N-acetylneuraminic acid, galactose and mannose-specific lectin were induced by CAR-Alum adjuvant, however, the affinity of IgG2b induced by CAR-Alum to GlcNAc and GalNAc-specific lectin were much less than that induced by Alum-CAR.