Journal of Radiation Protection and Research
- 제25권4호
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- Pages.223-232
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- 2000
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- 2508-1888(pISSN)
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- 2466-2461(eISSN)
FISH기법 및 단세포전기영동기법을 이용한 저선량 방사선에 의한 DNA 상해 및 염색체이상 평가
Assessment of DNA damage and Chromosome aberration in human lymphocyte exposed to low dose radiation detected by FISH(fluorescence in situ hybridization) and SCGE(single cell gel electrophoresis)
- 정해원 (서울대학교 보건대학원) ;
- 김수영 (서울대학교 보건대학원) ;
- 김병모 (서울대학교 보건대학원) ;
- 김선진 (서울대학교 보건대학원) ;
- 김태환 (원자력 병원) ;
-
조철구
(원자력 병원) ;
- 하성환 (서울대학교 의과대학)
- Chung, Hai-Won (School of Public health, Seoul National University) ;
- Kim, Su-Young (School of Public health, Seoul National University) ;
- Kim, Byung-Mo (School of Public health, Seoul National University) ;
- Kim, Sun-Jin (School of Public health, Seoul National University) ;
- Kim, Tae-Hwan (Korea Cancer Center Hospital) ;
-
Cho, Chul-Koo
(Korea Cancer Center Hospital) ;
- Ha, Sung-Whan (College of Medicine, Seoul National University)
- 발행 : 2000.12.30
초록
단세포전기영동법은 저선량 방사선에 의한 DNA손상을 민감하게 측정할 수 있는 방법으로 그 활용성이 증대되고 있다. 또한 각 염색체에 특이한 DNA probe를 이용하는 FISH기법은 염색체의 구조적 변화를 측정하는 매우 효과적인 방법으로서 그 유용성이 증대되고 있는 추세이다. 본 연구에서는 저선량 방사선의 측정을 위한 생물학적 선량계로서 FISH 기법과 단세포전기영동법의 활용가능성을 조사하고자 하였다. 5, 10, 30, 및 50 cGy의 저선량 방사선 조사에 의한 염색체이상빈도는 상호전좌의 경우 1, 2, 4번 염색체가 전체 genome상에서 차지하는 비율을 감안하여, 전체 세포수로 환산했을 때 cell equivalent당 0.0375, 0.0407, 0.0727 및 0.0814로 나타났으며, 이동원염색체의 경우 0.0125, 0.0174, 0.02291, 및 0.0407로 나타나 선량 증가에 따라 증가하는 것을 알 수 있었다. 또한
Comparative study was performed for the assessment of DNA damage and Chromosomal aberration in human lymphocyte exposed to low dose radiation using fluorescence in situ hybridization(FISH) and single cell gel electrophoresis(SCGE). Chromosomal aberrations in human lymphocytes exposed to radiation at doses of 5, 10, 30 and 50cGy were analysed with whole chromosome-specific probes by human chromosome 1, 2 and 4 according to PAINT system. FISH with chromosome-specific probe has been used to be a valid and rapid method fer detection of chromosome rearrangements induced by low dose radiation. The frequencies of stable translocation per cell equivalents were 0.0116, 0.0375, 0.040f, 0.0727 and 0.0814 for 0, 5, 10, 30 and 50cGy, respectively, and those of dicentric were 0.00, 0.0125, 0.174, 0.0291 and 0.0407 respectively. Radiation induced DNA damage in human lymphocyte in a dose-dependent manner at low doses from 5cGy to 50cGy, which were analysed by single tell gel electrophoresis(SCGE). From above results, FISH seemed to be useful for radiation biodosimetry by which the frequencies of stable aberrations in human lymphocyte can be observed more easily than by conventional method and SCGE also seemed to be sensitive method f9r detecting DNA damage by low dose radiation exposure, so that those methods will improve our technique to perform meaningful biodosimetry for radiation at low doses.
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