Thiamin Requirements for Vegetative Growth and Fruit Body Formation of Lentinula edodes

The effects of thiamin on vegetative mycelial growth and fruit body formation of Lentinuia edodes were investigated in basal peptone-glucose liquid medium in relation to the uptake of thiamin. Thiamin was essential for fruit body formation, and the minimum requirements for thiamin were estimated to be approximately 10 ${\mu}g$/L. The vegetative mycelial growth was little influenced by the addition of thiamin in the range of 1.5 ${\mu}g$~1.5 mg/L. While the mycelium was successively transferred to fresh peptone-glucose-agar medium three times, the repression of mycelial growth was not significant. Even in cases using vitamin-free casamino acid or glutamic acid as a nitrogen source instead of peptone, a thiamin deficiency for mycelial growth did not occur as a result of transferring the mycelia to fresh media. Almost all of the thiamin contained in the media accumulated in the mycelia during the first 3 weeks of a 9-week incubation. These results suggest that only trace amounts of thiamin are required for vegetative mycelial growth in Lentinula edodes and that almost all thiamin added to a basal medium will be used for fruit body formation.

표고버섯의 균사성장과 자실체 형성에 있어서 티아민의 효과를 펩톤 글루코스 기본 액체배지를 이용하여 조사하였다. 티아민은 표고버섯의 자실체 형성을 위해서는 필수 인자이며 최소 요구량은 10 ${\mu}g$/L 정도이다. 영양 생장기에는 티아민 농도 1.5 ${\mu}g$~1.5 mg/L의 범위 내에서는 영향을 거의 받지 않았다. 티아민을 첨가하지 않은 펩톤 글루코스 한천배지에 3회 계대 배양을 실시해도 티아민에 의한 균사성장의 차이는 나타나지 않았다. 이상의 결과, 티아민은 표고버섯의 균사성장에는 극히 소량이 요구되지만 거의 대부분의 티아민은 자실체 형성에 사용된다는 것을 알았다.