Ptotoplast Formation and Comparison of Phosphatase Activity between Intact Cell and Protoplast in Sccharomyces uvarum.

Sccharomyces uvarum의 protoplast 형성 및 intact cell과 protoplast의 phosphatase 활성도 비교

In this study, the stabilizer, PH and lysis method for optimum condition of S. uvarum protoplast formation were investigated, and also enzyme activity and poly-P formation of intact cell and protoplast mere compared. Upon protoplast formation, incubation time of 5 hours in snail gut enzyme and 3 hours in drisielase were reignited. 0.8 Mole mannitol and 6 mole KCl were apt to protoplast formation. Protoplast was contained less 22-27 percentage in ALPase, 4-15 percentage in ACPase than intact cell. Accumulation of inorganic polyphosphate did not increase significently in protoplast compared with intact cell.

효모세포의 원형질체 최적형성을 위한 stabilizer의 종류 및 농도, pH 그리고 lysis 방법을 조사하는 한편, intact cell과 protoplast사이의 효소활성도 및 poly-P 생합성율을 비교하였다. 그 결과 protoplast 형성에 있어 snail gut enzyme은 5시간, drisielase는 3시간 정도의 incubation 시간이 필요했으며, stabilizer로는 0.8 M mannitol, 6 M KCl이 좋았다. Protoplast는 intact cell에 비해 ALPase 활성은 22-27%, ACPase는 4-15% 정도 감소하였으며, poly-P 형성은 protoplast에서 유의한 증가가 일어나지 않았다.