Journal of Dairy Science and Biotechnology

Korean Society of Dairy Science and Biotechnology (한국낙농식품응용생물학회)
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Natural Antibiotic Activity of Lactobacillus helveticus

Lactobacillus helveticus의 자연항생작용에 관한 연구

  • Choi, Sang-Hun (Department of Animal Science, Kyungpook National University) ;
  • Kim, Dong-Sin (Department of Animal Science, Kyungpook National University)
  • 최상훈 (경북대학교 동물자원학과) ;
  • 김동신 (경북대학교 동물자원학과)
  • Published : 1999.05.28
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Abstract

The objective of this study was to extract and purity the antibacterial agent from the fermented milk with Lactobacillus helveticus CH-1. The extraction and purification of antibacterial agent from the Lb. helveticus fermented milk were carried out by methanol extraction, acetone extraction, Sephadex G-200 gel filteration and thin layer chromatography and the results were as followings. The antibacterial activity of methanol-acetone extraction showed antibacterial activity against test organisms, B. subtilis, E. coli, Pseu. fluorescens, Sal. typhimurium, Shi. flexneri, and Sta. aureus. Sephadex G-200 gel chromatography showed only antibacterial activity from 33 to 37th fractions of 60 fractions. The agent purified from TLC plate confirmed the antibacterial activity by the means of bioautography.

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