대한골관절종양학회지 (The Journal of the Korean bone and joint tumor society)
- 제5권1호
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- Pages.1-8
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- 1999
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- 1226-4962(pISSN)
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- 2233-9841(eISSN)
고용량 방사선 조사 후 골육종 세포주(Saos-2)의 아포프토시스 발생
Induction of Apoptosis in Human Osteosarcoma Cell Lines(Saos-2) by Single Fraction High Dose Irradiation
- 김재도 (고신대학교 복음병원 정형외과학교실) ;
- 정소학 (고신대학교 복음병원 정형외과학교실) ;
- 홍영기 (고신대학교 복음병원 정형외과학교실) ;
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최장석
(인제대학교 부산백병원 정형외과학교실)
- Kim, Jae-Do (Department of Orthopaedic Surgery, Gospel Hospital, Kosin University) ;
- Chung, So-Hak (Department of Orthopaedic Surgery, Gospel Hospital, Kosin University) ;
- Hong, Young-Gi (Department of Orthopaedic Surgery, Gospel Hospital, Kosin University) ;
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Choi, Jang-Seok
(Department of Orthopaedic Surgery, Pusan Baek Hospital, Inje University)
- 발행 : 1999.03.30
초록
A single fraction of 50 Gy extracorporeal irradiation, as a modality of limb-sparing operation, has been used to achieve tumor necrosis in osteosarcoma. Although this modality of radiation therapy preserving the mobility of a joint is commonly practiced, the precise knowledge on the radiobiological response of osteosarcoma cell has remained to be elucidated. We therefore observed whether a single high dose irradiation caused apoptosis in osteosarcoma cells and whether the commitment to apoptosis was associated with cell kinetics. We also investigated radiation dose response along the time course for development of apoptosis following single high dose irradiation. The morphologic change in apoptosis was observed by fluorescence with Hoechst 33258 and the degree and the fraction of cells by flow cytometry. Irradiation of osteosarcoma cells with 10, 30 and 50 Gy resulted in chromatin condensation and apoptotic body formation. The degree of apoptosis in osteosarcoma cells was