Maxillofacial Plastic and Reconstructive Surgery
- Volume 21 Issue 3
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- Pages.231-239
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- 1999
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- 2288-8101(pISSN)
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- 2288-8586(eISSN)
CULTURED HUMAN ORAL KERATINOCYTES; ULTRASTRUCTURAL STUDY
배양된 사람치은각화상피세포의 미세구조
- Kwon, Yong-Dae (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyung-Hee University) ;
- Lee, Baek-Soo (Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyung-Hee University) ;
- Jue, Sung-Sook (Department of Oral Anatomy, College of Dentistry, Kyung-Hee University)
- Received : 1999.05.17
- Accepted : 1999.06.22
- Published : 1999.09.30
Abstract
In oral and maxillofacial surgery, there are many cases requiring the graft of epidermal tissues such as maxillectomy, and vestibuloplasty. There have been so many challenges for the culture of the epidermal tissue. Observing the ultrastructure of the cultured human oral kertinocytes, we could compare this findings with that of in vivo ones. With that, we could find the differencies and similarities between cultured cells and in vivo ones, and evaluate the clinical applications of cultured tissue. Human gingiva was obtained and the specimen was explanted on 24-well plate. Two types of culture media were used in this culture system. One was for the growth of the keratinocytes (Media I), and the other was for the stratification (Media II). Media I had special ingredients for the epidermal growth. Those were 0.5% dimethyl sulfoxide (DMSO), 30ng/ml of epidermal growth factor (EGF), 30ng/ml of cholera toxin, and
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