Restorative Dentistry and Endodontics
- Volume 24 Issue 4
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- Pages.535-545
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- 1999
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- 2234-7658(pISSN)
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- 2234-7666(eISSN)
VARIATION OF UREASE GENES(ureC) FROM Streptococcus salivarius
Streptococcus salivarisu의 요소분해효소 유전자 변이에 관한 연구
- Choi, Hye-Jin (Department of Conservative Dentistry, College of Dentistry, Kyung Hee University) ;
- Lee, Jin-Yong (Department of Conservative Dentistry, College of Dentistry, Kyung Hee University) ;
- Choi, Ho-Young (Department of Conservative Dentistry, College of Dentistry, Kyung Hee University)
- Published : 1999.12.02
Abstract
Crease of Streptococcus salivarius is believed to play a critical role in bacterial ecology and pH homeostasis in the mouth, and consequently affect the pathogenesis of dental caries and periodontal diseases. Expression of the urease gene is greatly enhanced by low p. f. excess of Carbohydrate, and faster growth. It was observed that urease activity of the strains of S. salivarius that exhibited no of low urease activity was not increased even in low pH condition. In this study, it was hypothesized that the urease gene of the strains is absent, defected, or greatly changed by genetic combination. In order to prove this hypothesis, chromosomes were obtained from 28 S. salivarius strains which had been isolated from normal teeth and carious lesions, subjected to polymerase chain reaction (PCR) using primers encoding highly conserved sequence from ureC, and then the obtained PCR products were compared. The results were as follows: 1. After PCR the strains generated either one of 0.54- and 1.3-kbp PCR products, or none. 2. All 16 strains having a higher urease activity(<50
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