The Growth of Proteolytic Bacteria Immobilized in Capsule Type

  • Han Bong-Ho (Dept. of Food Science and Technology, Pukyong National University) ;
  • Choi Su-Il (Dept. of Food Technology, Tong Myong College) ;
  • Kim Seong-Bong (Dept. of Food Science and Technology, Pukyong National University) ;
  • Kim Sang-Ho (Dept. of Food Science and Technology, Pukyong National University)
  • Published : 1999.06.01

Abstract

Proteolytic bacteria isolated from fermented anchovy jeotkal were immobilized in capsule type with $0.8\%$ sodium alginate and $CaCl_2/carboxymethyl$ cellulose (CMC). For making the immobilized capsule, the optimal concentration of both $CaCl_2$ and CMC, with respect to the membrane hardness and the growth of proteolytic bacteria in capsule, were $2.0\%$ at following conditions: flow rate of $CaCl_2/CMC$ solution and cell suspension were respectively 3.54 ml/min and 0.15 ml/min when inside diameter of inner and outer capillary tube in immobilizing apparatus were 0.32mm, 0.74mm, respectively. The density of proteolytic bacteria in capsule reached maximum, i.e. $10^8-10^9cells$/capsule during culture under optimal conditions in TPY broth, and these were $10^2-10^4$ times higher than these of before culture. During culture of proteolytic bacteria immobilized in capsule type (PBImC) for 72hrs, few growing cells were lost in the outer medium.

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