Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Establishment of a diagnostic method for porcine proliferative enteropathy using polymerase chain reaction

중합효소연쇄반응을 이용한 돼지 증식성 장염 진단기법 확립

  • Lym, Suk-kyung (National Veterinary Research & Quarantine Service, Ministry of Agriculture & Forestry) ;
  • Lee, Hee-soo (National Veterinary Research & Quarantine Service, Ministry of Agriculture & Forestry) ;
  • Woo, Sung-ryong (National Veterinary Research & Quarantine Service, Ministry of Agriculture & Forestry) ;
  • Yoon, Soon-seek (National Veterinary Research & Quarantine Service, Ministry of Agriculture & Forestry) ;
  • Moon, Oun-kyong (National Veterinary Research & Quarantine Service, Ministry of Agriculture & Forestry) ;
  • Lee, Yoo-young (National Veterinary Research & Quarantine Service, Ministry of Agriculture & Forestry) ;
  • Koh, Hong-bum (College of Veterinary Medicine, Chonnam National University)
  • 임숙경 (농림부 국립수의과학검역원) ;
  • 이희수 (농림부 국립수의과학검역원) ;
  • 우승룡 (농림부 국립수의과학검역원) ;
  • 윤순식 (농림부 국립수의과학검역원) ;
  • 문운경 (농림부 국립수의과학검역원) ;
  • 이유영 (농림부 국립수의과학검역원) ;
  • 고홍범 (전남대학교 수의과대학)
  • Received : 1988.12.02
  • Published : 1999.03.22
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Abstract

Porcine Proliferative Enteropathy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 to 20 weeks of age. PPE has been diagnosed by clinical signs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs. Therefore, in this study, we established the polymerase chain reaction(PCR) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonuclease analysis with restriction enzyme Hae III and Pst I. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.

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