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Enzymatic Glycosylation of Fatty Acids by Methyl Glycosides

메틸글리코시드에 의한 지방산의 효소적 배당화

  • SunWoo, Hwan (Department of Chemical Engineering, Myong Ji University) ;
  • Kim, Chong-Tai (Food Engineering & Marketing Div., Korea Food Research Institute) ;
  • Kim, Hae-Sung (Department of Chemical Engineering, Myong Ji University)
  • 선우환 (명지대학교 화학공학과) ;
  • 김종태 (한국식품개발연구원 산업화연구부) ;
  • 김해성 (명지대학교 화학공학과)
  • Published : 1999.03.31

Abstract

Glycoside fatty acid esters were synthesized by lipase-catalyzed glycosylation of fatty acids with methyl glycoside in solvent and solvent free process. Optimum condition of solvent process using 2-methyl-2-propanol were : moral ratio of methyl glycoside to fatty acid 1:3: initial concentration of methyl glycoside 50g/l:enzyme(immodilized lipase Novozym 435 from Candidia antarctica) content 1%(w/v) : desiccant content 9%(w/v); reaction temperature $60^{\circ}C$: reaction time 10hrs. The yield of 99% was obtained. Solvent-free process was carried out in total absence of solvent at $70^{\circ}C$ under reduced pressure, 5-20mmHg. To give meximum yield of 99% at the optimum condition of molar ratio of methyl glycoside to fatty acid 1:3, enzyme content 10%(w/w), and reaction time 10hrs. The glycosylation reactivity of different glycosylation agents were sequent to $Methyl-{\beta}-D-fructofuranoside$. $Methyl-{\beta}-D-glucopyranoside$. $Methyl-{\beta}-D-fructofuranosi$ de, and $Methyl-{\alpha}-D-glucopyranoside$.

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