Journal of Periodontal and Implant Science
- Volume 29 Issue 4
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- Pages.785-804
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- 1999
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- 2093-2278(pISSN)
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- 2093-2286(eISSN)
Effects of Platelet-derived Growth Factor on the Activity of Osteoblastic Cells
Platelet-derived growth factor가 조골세포의 활성에 미치는 영향
- Choi, Hyoung-Ho (Department of Periodontology, College of Dentistry, Dan-Kook University) ;
- Kim, Jung-Keun (Department of Periodontology, College of Dentistry, Dan-Kook University) ;
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Lim, Sung-Bin
(Department of Periodontology, College of Dentistry, Dan-Kook University) ;
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Chung, Chin-Hyung
(Department of Periodontology, College of Dentistry, Dan-Kook University)
- 최형호 (단국대학교 치과대학 치주과학교실) ;
- 김정근 (단국대학교 치과대학 치주과학교실) ;
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임성빈
(단국대학교 치과대학 치주과학교실) ;
-
정진형
(단국대학교 치과대학 치주과학교실)
- Published : 1999.12.31
Abstract
The cell activities of bone metabolism is affected by growth factor rather than by hormone. The affects of growth factors on the bone activity were observed using various culture methods. Platelet-derived growth factor(PDGF) is produced from the well differentiated bone cell. It stimulates cell mitosis, synthesizes collagen in bone tissue and plays a role in healing response. The purpose of this study is to evaluate the effects that PDGF has on the activity and the proliferation of osteoblast by measuring the activity of alkaline phosphatase, the growth formation of calcified nodules, and osteocalcin production. In this study, HOS and ROS 17/2.8 osteoblastic cell line was used, along with variable concentrations of PDGF the were measured with osteoblastic proliferation. The cell proliferation of HOS and ROS 17/2.8 cells was stimulated dose- depentdently. Alakline phosphatase activity was significantly decreased by PDGF in osteoblastic cells. A number of small calcified nodules were observed in HOS cell treated with low concentrations(0.1, 0.4 ng/ml) of PDGF-BB and no significant difference from control group was found. High concentrations(10, 50 ng/ml) of PDGF suppressed calcified nodule formation. And osteocalcin production was inhibited with PDGF. These results suggest that PDGF stimulates the osteoblastic proliferation, whereas suppresses the individual cellular functions.
Keywords