The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Purification Siderophore from Vibrio mimicus ATCC 33653 and its Effect to Bacterial Pathogenecity

Vibrio mimicus ATCC 33653이 생산하는 Siderophore의 정제와 균의 병원성에 미치는 영향

  • Park, Soo-Jung (Department of Microbiology, Graduate School, Pusan National University) ;
  • Ju, Seong-A (Division of biological Sciences, College of Natural Science, Ulsan University) ;
  • Heo, Moon-Soo (Faculty of Applied Marine Sciences, College of Ocean Sciences, Cheju National University) ;
  • Jung, Cho-Rok (Department of Microbiology, Graduate School, Pusan National University) ;
  • Ju, Jin-Woo (Department of Microbiology, College of Natural Science, Pusan National University)
  • 박수정 (부산대학교 자연과학대학 대학원 미생물학과) ;
  • 주성아 (울산대학교 자연과학대학 생명과학부) ;
  • 허문수 (제주대학교 해양과학대학 응용해양과학) ;
  • 정초록 (부산대학교 자연과학대학 대학원 미생물학과) ;
  • 주진우 (부산대학교 자연과학대학 미생물학과)
  • Published : 1999.10.30
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Abstract

Growth under conditions of iron-restriction and the production of siderophore was examined in Vibrio mimicus ATCC 33653. This strain grew and multiplied in the presence of the high-affinity iron chelators ethylenediamine-di (o-hydroxyphenylacetic acid). Chrome azurol S (CAS) agar and solution were used to detect the production of siderophore under these condition. Siderophore could be detected in the iron-restricted culture supernatants. The siderophore was extracted from iron-restricted culture supernatants by phenol-chloroform-ether method and purified by Dowex ion-exchange and Sephadex G-25 gel filtracton chromatography. The purified siderophore was confirmed by paper chromatography and HPLC. The Purified siderophore enhanced the growth of V. mimicus when the bacterium was grown in iron limited medium. Injection of both the siderohore and the bacteria to mice resulted in more rapid death than that of the only bacteria. However, the siderophore did not show lethality to mice and any toxicity to cell line like HeLa and U937.

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