The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Infectivity of Orientia tsutsugamushi to Various Eukaryotic Cells and Their Cellular Invasion Mechanism

Orientia tsutsugamushi의 유핵세포내 감염능 분석 및 기전

  • Ihn, Kyung-Soo (Department of Microbiology, Seoul National University College of Medicine) ;
  • Han, Seung-Hoon (Department of Microbiology, Seoul National University College of Medicine) ;
  • Kim, Hang-Rae (Department of Microbiology, Seoul National University College of Medicine) ;
  • Seong, Seung-Yong (Department of Microbiology, Seoul National University College of Medicine) ;
  • Kim, Ik-Sang (Department of Microbiology, Seoul National University College of Medicine) ;
  • Choi, Myung-Sik (Department of Microbiology, Seoul National University College of Medicine)
  • 인경수 (서울대학교 의과대학 미생물학교실) ;
  • 한승훈 (서울대학교 의과대학 미생물학교실) ;
  • 김항래 (서울대학교 의과대학 미생물학교실) ;
  • 성승용 (서울대학교 의과대학 미생물학교실) ;
  • 김익상 (서울대학교 의과대학 미생물학교실) ;
  • 최명식 (서울대학교 의과대학 미생물학교실)
  • Published : 1999.10.30
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Abstract

Orientia tsutsugamushi is obligate intracellular bacterium that grows within the cytoplasm of the eukaryotic host cells. Therefore capability of the attachment, entry into the host cell and intracellular survival should be critical process for oriential infection. In this study we investigated the cellular invasion mechanism of Orientia tsutsugamushi and the role of transmembrane heparan sulfate proteoglycan, which binds diverse components at the cellular microenvironment and is implicated as host cell receptors for a variety of microbial pathogens. First of all Orientia tsutsugamushi can invade a wide range of nonprofessional phagocytic cells including fibroblast, epithelial cells and endothelial cells of various host species, including Band T lymphocytes. Thus, it was postulated that the attachment of O. tsutsugamushi requires the recognition of ubiquitous surface structures of many kinds of host cells. Treatments with heparan sulfate and heparin inhibited the infection of Orientia tsutsugamushi in dose-dependent manner for L cell, mouse fibroblast, whereas other glycosaminoglycans such as chondroitin sulfate had no effect. Collectively, these findings provide strong evidence that initial interaction with heparan sulfate proteoglycan is required for the oriential invasion into host cells.

Keywords

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