KSBB Journal
- Volume 14 Issue 6
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- Pages.713-717
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- 1999
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- 1225-7117(pISSN)
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- 2288-8268(eISSN)
Properties of Carbohydrase Prepared from Lipomyces starkeyi JLC26
Lipomyces starkeyi JLC26에서 유래된 Carbohydrase의 특성
- Jun, Sun-Mee (Department of Biomedical Engineering, Chonnam National University) ;
- Kim, Do-Man (Biochemical Engineering and the Research Institute for Catalysis, Chonnam National University, Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
- Kim, Do-Won (Department of Physics, Kangnung National University)
- Published : 1999.12.01
Abstract
We have isolated a dextranase and amylase constitutive and hyper-producing mutant, Lipomyces starkeyi JLC26, from Lipomyces starkeyi ATCC74054 after mutation using UV irradiation. After partial purification of dextranase and amylase (together DXAMase;both activities were always co-purified) by ammonium sulfate precipitation, CM-Sepharose column chromatography, the specific activities of amylase and dextranase were 5367 and 3045 unit/mg, respectively. The pH effects for activity and stabiligy of both enzymes were similar to each other: Optimum pH and temperature for activity sere at 5.5 and 37
Lipomyces starkeyi ATCC 74054를 UV로 돌연변이하여 선발한 JLC26의 효소적인 특성을 알아보았다. 효소는 70% ammonium sulfate로 침전시키고, CM-Sepharose column chromatography로 부분 정제하였다. 이때 얻어진 각 효소 specific activity는 amylase 활성의 경우는 5367 unit/mg이었고 dextranase 활성의 경우는 3045 unit/mg이었다. 효소의 최적 pH와 온도는 5.5와 37