Cultural Condition and Morphological Charateristics of Paecilomyces Japonica for the Artificial Cultivation

눈꽃동충하초(Paecilomyces japonica Yasuda)의 형태 및 배양조건

The optimal condition for the production of the artificial fruiting bodies of Paecilomyces japonica in bulk was determined from the observation of the conidial, hyphal, and cultural condition and the medium. The colony was grown 32 mm diameter in 14 days on potato dextrose agar(PDA). Conidia was irregularly long oval-shaped and measured 4.07${\times}$1.56 ${\mu}$m in average. The hyaline hyphae formed transparent bundles which branched out. The fruiting body on the insect surface was measured 30 to 50 mm in length and formed up to 55 branches. Potato dextrose(PD) both was the most effective for the growth of Paecilomyces japonica among the liquid type media. About 3.1 mg mrcelia in dry weight were produced in 50 ml/PD broth. The best condition for the production of conidia under which condition 4.3${\times}$10$^8$ conidia/ml were harvested was pH 5.0 in acidity and 20$^{\circ}C$ following preculture at 24$^{\circ}C$ in temperature for 7 days.

눈꽃 동총하초의 인공재배를 위한 최적배양조건을 구명하기 위해 본 시험을 수행하였으며 그 결과 colony는 직경 32 mm/14 days로 생장하였으며 분생포자는 불규칙한 장타원형으로 평균 4.07${\times}$1.56 ${\mu}$m 크기이며 분생자병은 투명다발의 분자형으로 발생하였다. 자실체는 지상부에서 30~50 mm로 다발형태로 발생하며 평균 55개의 분지를 형성한다. 종균배양 액체배지 선발결과 PD배지가 건중량 3.15${\pm}$0.16 mg/50 m/로 균사생장에 가장 효과가 우수하였다. 분생포자 생산 최적 환경조건은 산도 pH 5.0 온도는 24$^{\circ}C$에서 20$^{\circ}C$로 변온을 가한 경우 4.30${\pm}$0.35${\times}$10$^8$ conidia/m/로 기타 처리구에 비해 포자형성율이 가장 우수하였다.