Journal of Sericultural and Entomological Science (한국잠사곤충학회지)
- Volume 41 Issue 1
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- Pages.1-8
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- 1999
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- 2234-8174(pISSN)
Electrophoretic Patterns of Hemolymph Proteins of Varieties with Long and Short Life Span in the Silkworm Bombyx mori L.
장${\cdot}$ 단명 누에 품종의 체액단백질의 전기영동상
- Kang, Pil-Don (National Institute of Agricultural Science & Technology Department of Sericulture & Entomology, RDA.) ;
- Yoon, Hyung-Joo (National Institute of Agricultural Science & Technology Department of Sericulture & Entomology, RDA.) ;
- Ryu, Kang-Sun (National Institute of Agricultural Science & Technology Department of Sericulture & Entomology, RDA.) ;
- Sohn, Bong-Hee (National Institute of Agricultural Science & Technology Department of Sericulture & Entomology, RDA.) ;
- Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University)
- 강필돈 (농촌진흥청 농업과학기술원 잠사곤충부) ;
- 윤형주 (농촌진흥청 농업과학기술원 잠사곤충부) ;
- 류강선 (농촌진흥청 농업과학기술원 잠사곤충부) ;
- 손봉희 (농촌진흥청 농업과학기술원 잠사곤충부) ;
- 손흥대 (동아대학교 생명자원과학대학)
- Published : 1999.06.01
Abstract
Hemolymph protein patterns of silkworms in terms of short and long life span were analyzed by native- and SDS-polyacrylamide gel electrophoresis (PAGE) with the developmental stages. From the native-PAGE patterns of silkworm major hemolymph proteins there were varietal differences on the first day of the pupal stage and were classified into there groups MHP-a, b and c SA 10, JAM109 and J037 were grouped into MHP-Ⅰ, Hangang, Chungmun and Daizo(sdi) into MHP-Ⅱ and NTZN, Sulak, Qoichuk and PR varieties into MHP-Ⅲ group. It was found that the MHP in each group revealed similar patterns and changes with development of pupal stage. In the first day of adult, MHP-c was clearly detected in both female and male of the Daizo(sdi), but not in the J037, indicating that there was significantly varietal differences in electrophoretical protein pattern. In addition, the results of protein pattern of hemolymph by SDS-PAGE showed also varietal differences in the concentration of hemolymph protein.
장명품종(SA10, NTZN, 잠109, J037 및 설악)과 단명품종[한강, 괴축, PR, 천문 및 Daizo(sdi)]의 幼蟲, 용, 成蟲의 발육단계별 및 암수별 체액단백질의 변화과정을 전기영동상의 패턴에 의해 비교 분석하였다. Native 전기영동상 분석 결과로 볼 때, 용 1일째의 體液 蛋白質은 MHP-a, b, c 패턴에 따라 長命特異群(MHP-Ⅰgroup), 短命特異群(MHP-Ⅱgroup) 및 張