대한바이러스학회지 (The Journal of Korean Society of Virology)
- 제29권2호
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- Pages.119-127
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- 1999
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- 1225-2344(pISSN)
한국인 HIV 감염자에서 분리된 HIV-1 Subtype A의 env 유전자 V3-V5 부위의 계통적 분석
Phylogenetic Analysis of env Gene V3-V5 Region of HIV-1 Subtype A Isolates from Korean
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이주실
(국립보건원 바이러스질환부 면역결핍연구실) ;
- 김은영 (국립보건원 바이러스질환부 면역결핍연구실) ;
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강춘
(국립보건원 바이러스질환부 면역결핍연구실) ;
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남정구
(국립보건원 바이러스질환부 면역결핍연구실) ;
- 이성래 (국립보건원 바이러스질환부 면역결핍연구실) ;
- 구본기 (국립보건원 바이러스질환부 면역결핍연구실) ;
- 신영오 (국립보건원 바이러스질환부 면역결핍연구실)
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Lee, Joo-Shil
(Center for AIDS Research, Department of Virology, National Institute of Health) ;
- Kim, Eun-Young (Center for AIDS Research, Department of Virology, National Institute of Health) ;
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Kang, Chun
(Center for AIDS Research, Department of Virology, National Institute of Health) ;
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Nam, Jeong-Gu
(Center for AIDS Research, Department of Virology, National Institute of Health) ;
- Lee, Sung-Rae (Center for AIDS Research, Department of Virology, National Institute of Health) ;
- Koo, Bon-Ki (Center for AIDS Research, Department of Virology, National Institute of Health) ;
- Shin, Yung-Oh (Center for AIDS Research, Department of Virology, National Institute of Health)
- 발행 : 1999.06.30
초록
Phylogenetic analysis was conducted to monitor transmission of HIV and to investigate the genetic structure of primary isolates from 12 HIV-1 subtype A infected Koreans. The individuals infected with subtype A viruses had been diagnosed as HIV-1 seropositives during the period 1987 to 1995 and blood samples have been collected from 1991 to 1997. DNA of each individual was isolated from uncultured or cultured peripheral blood mononuclear cells. V3-V5 (0.7 kb) fragment of HIV-1 env gene was amplified by nested polymerase chain reaction and the PCR products were sequenced. The mean value of the divergence of nucleotide of HIV-1 env V3-V5 fragment was