대한바이러스학회지 (The Journal of Korean Society of Virology)
- 제29권2호
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- Pages.99-105
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- 1999
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- 1225-2344(pISSN)
Detection of Glycoproteins (B and D) and Thymidine Kinase Genes of Herpes simplex virus Type 2 Strain G
- Kang, Hyun (Department of Biological Science, Konkuk University) ;
- Park, Jong-Kuk (Department of Biological Science, Konkuk University) ;
- Uh, Hong-Sun (Department of Biological Science, Konkuk University) ;
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Kim, Soo-Young
(Department of Biological Science, Konkuk University) ;
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Lee, Hyung-Hoan
(Department of Biological Science, Konkuk University)
- 발행 : 1999.06.30
초록
BamHI restriction pattern and genomic library of Herpes simplex virus type 2 (HSV-2) strain G were constructed, and locations of the glycoproteins gB and gD, and tk genes on the fragments were detected by Southern blot analysis. HSV-2 genomic DNAs were cleaved into twenty-seven fragments by BamHI enzyme in the range of 0.72 to 15.08 (total 150.44 kb), which were cloned into the BamHI site of pBluescript SK(+) to construct genome library of the HSV-2. The library was named by the order of the fragment size from smallest one to largest one. HSV-2 glycoprotein gD gene was located in pHLA2-21 and pHLA2-22 recombinant plasmids, gB gene in pHLA2-24 plasmid, and tk gene in pHLA2-11 clone by Southern blot analysis.
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