Isolation and Characterization of Reovirus in Korea

한국에 분포하는 레오바이러스의 분리 및 동정

  • Song, Ki-Joon (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University) ;
  • Kang, Byung-Chul (Graduate School, Korea University) ;
  • Lee, Young-Eun (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University) ;
  • Baek, Luck-Ju (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University) ;
  • Lee, Yong-Ju (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University) ;
  • Song, Jin-Won (Department of Microbiology, College of Medicine, Institute for Viral Disease, Korea University)
  • 송기준 (고려대학교 의과대학 미생물학교실, 바이러스병연구소) ;
  • 강병철 (고려대학교 대학원) ;
  • 이영은 (고려대학교 의과대학 미생물학교실, 바이러스병연구소) ;
  • 백락주 (고려대학교 의과대학 미생물학교실, 바이러스병연구소) ;
  • 이용주 (고려대학교 의과대학 미생물학교실, 바이러스병연구소) ;
  • 송진원 (고려대학교 의과대학 미생물학교실, 바이러스병연구소)
  • Published : 1999.06.30

Abstract

Reovirus was found to inhabit both the respiratory and the enteric tract of human and animals. The genome of reovirus comprises 10 segments of double-stranded RNA, total size 24 kbp. Nine strains of reovirus were isolated from human and field mice in Korea. Aseptically collected sera from human and lung tissues from field mice were used for virus isolation. For serotype determination, hemagglutination inhibition test was used, and three strains were confirmed to type 2 and six strains to type 3. To determine the genomic diversity and molecular phylogeny of reoviruses isolated in Korea, part of S4 genomic segment of reovirus was enzymatically amplified and directly sequenced. In nucleotide level, Apo98-35 strain showed 15.4%, 19.3%, and 14.4% differences compared to type 1 (T1L, Lang), type 2 (T2J), and type 3 reference strains, respectively. In amino acid level, Apo98-35 strain showed 10.5%, 13.7%, and 9.5% differences compared to type 1, type 2, and type 3 reference strains, respectively. Using the maximum parsimony method based on 285 bp spaning region of the S4 genomic segment, phylogenetic analysis indicated that Apo98-35 from Korea formed different phylogenetic branch. Our data obtained by sequence and phylogenetic analyses of reoviruses are consistent with the distinct geographically dependent evolution of reoviruses in Korea.

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