The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Isolation, identification, and culture conditions of the strain producing antibacterial antibiotic

항세균성 항생물질을 생산하는 미생물의 분리, 동정 및 배양조건

  • Yoo, Jae-Hong (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Yoon, Sang-Hong (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Koo, Bon-Sung (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Yeo, Yun-Soo (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Park, In-Cheol (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Byung-Moo (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA) ;
  • Ryu, Jin-Chang (Dept. of Molecular Genetics, National Institute of Agricultural Science and Technology, RDA)
  • 유재홍 (농업과학기술원 생물자원부) ;
  • 윤상홍 (농업과학기술원 생물자원부) ;
  • 구본성 (농업과학기술원 생물자원부) ;
  • 여윤수 (농업과학기술원 생물자원부) ;
  • 박인철 (농업과학기술원 생물자원부) ;
  • 이병무 (농업과학기술원 생물자원부) ;
  • 류진창 (농업과학기술원 생물자원부)
  • Published : 1999.08.25
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Abstract

The strain with antibacterial activity was isolated among soil samples collected in Suwon area. The isolated strain was identified as Bacillus sp. YR-1 with respect to its morphological, cultural, and physiological characteristics. Optimal medium for the highest production of antibiotic was composed of sucrose 2.0%(w/v), peptone 2.0%(w/v) and NaCl 0.1%(w/v). The maximum production of antibiotic was shown at $35^{\circ}C$ for 48 hours with the initial pH 7.0.

식물에 세균성무름병(soft rot disease)과 검은반점병(blackleg)을 일으키는 Erwinia carotovora subsp. carotovora의 생육을 저해하는 균주를 토양으로부터 분리하고, 그 중 항균 활성이 가장 좋은 균주를 선별하여 균주의 동정, 항생물질의 최적 생산조건 및 생산된 항생물질의 특성을 연구 검토한 결과 다음과 같은 결론을 얻었다. 분리 균주의 형태학적, 배양학적 및 생리학적 특성을 검토한 결과 Bacillus sp.로 판단되어 분리균주를 Bacillus sp. YR-1로 명명하였다. 항생물질 생산을 위한 최적 조건을 검토한 결과, sucrose 2.0%(w/v), peptone 2.0%(w/v), NaCl 0.1%(w/v), pH 7.0의 배지 $35^{\circ}C$, 48시간 진탕배양 하였을 때, 항생물질의 생산성이 가장 좋았다.

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