Clinical and Experimental Reproductive Medicine
- 제26권1호
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- Pages.103-109
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- 1999
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
비정상적 정자형성 환자의 정소에서 Heat Shock Protein A2 (hspA2) mRNA 발현의 감소
Repression of HspA2 mRNA Expression in Human Testes with Abnormal Spermatogenesis
- 손원영 (대진의료재단 분당 제생병원 불임 및 생식의학연구소) ;
- 황서하 (서강대학교 이과대학 생명과학과) ;
- 한징택 (서강대학교 이과대학 생명과학과) ;
- 이재호 (대진의료재단 분당 제생병원 불임 및 생식의학연구소) ;
- 김석중 (대진의료재단 분당 제생병원 불임 및 생식의학연구소) ;
- 김영찬 (대진의료재단 분당 제생병원 불임 및 생식의학연구소)
- Son, W.Y. (Center for Reproduction and Genetics, Pundang Jesaeng General Hospital) ;
- Hwang, S.H. (Department of Life Science, Sogang University) ;
- Han, C.T. (Department of Life Science, Sogang University) ;
- Lee, J.H. (Center for Reproduction and Genetics, Pundang Jesaeng General Hospital) ;
- Kim, S.J. (Center for Reproduction and Genetics, Pundang Jesaeng General Hospital) ;
- Kim, Y.C. (Center for Reproduction and Genetics, Pundang Jesaeng General Hospital)
- 발행 : 1999.03.30
초록
Objective: Heat shock protein 70-2 (Hsp70-2) gene knockout mice are found to have premeiotic arrest at the primary spermatocyte stage with a complete absence of spermatids and spermatozoa. This observation led to the hypothesis that hspA2 may be disrupted in human testes with abnormal spermatogenesis. To test this hypothesis, we studied the mRNA expression of hspA2 in infertile men with azoospermia. Design: The mRNA expression were analyzed by competitive RT-PCR among testes with normal spermatogenesis, pachytene spermatocyte arrest, and sertoli-cell only syndrome. Materials and methods: Testicular biopsy was performed in men with azoospermia (n=15). Specimens were subdivided into three groups: (group 1) normal spermatogenesis (n=5), (group 2) spermatocyte arrest (n=5), (group 3) Sertoli-cell only syndrome (n=5). Total RNA was extracted by Trizol reagent. Total extracted RNA was reverse transcribed into cDNA and amplified by PCR using specific primers for hspA2 target cDNAs. A competitive cDNA fragment was constructed by deleting a defined fragment from the target cDNA sequence, and then coamplified with the target cDNA for competitive PCR. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was used as an internal control. Results: On Competitive RT-PCR analyses for hspA2 mRNA, significant amount of hspA2 expression was observed in group 1, whereas a constitutively low level of hspA2 was expressed in groups 2 and 3. Conclusion(s): The study demonstrates that the hspA2 gene expression is down-regulated in human testes with abnormal spermatogenesis, which in turn suggests that hspA2 gene may play a specific role during meiosis in human testes.