Clinical and Experimental Reproductive Medicine
- 제26권1호
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- Pages.89-96
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- 1999
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Streptozotocin으로 유발된 당뇨병성 Wistar Rat 정자의 첨체반응 및 수 변화 특성
Spermatozoa Characteristics of Streptozotocin-induced Diabetic Wistar Rat: Acrosome Reaction and Spermatozoa Concentration
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전용필
(울산대학교 아산재단 서울중앙병원 산부인과학교실) ;
- 김정훈 (울산대학교 아산재단 서울중앙병원 산부인과학교실) ;
- 강병문 (울산대학교 아산재단 서울중앙병원 산부인과학교실) ;
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장윤석
(울산대학교 아산재단 서울중앙병원 산부인과학교실) ;
- 남주현 (울산대학교 아산재단 서울중앙병원 산부인과학교실) ;
- 김영수 (충남대학교 의과대학 비뇨기과학교실) ;
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계명찬
(경기대학교 자연과학대학 생물학과) ;
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김문규
(한양대학교 자연과학대학 생물학과) ;
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김길수
(아산생명과학연구소 실험동물연구실)
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Cheon, Yong-Pil
(Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center) ;
- Kim, Chung-Hoon (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center) ;
- Kang, Byung-Moon (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center) ;
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Chang, Yoon-Seok
(Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center) ;
- Nam, Joo-Hyun (Department of Obstetrics and Gynecology, College of Medicine, University of Ulsan, Asan Medical Center) ;
- Kim, Young-Soo (Department of Urology, College of Medicine, Choongnam University) ;
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Gye, Myung-Chan
(Department of Biology, College of Natural Sciences, Kyonggi University) ;
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Kim, Moon-Kyoo
(Department of Biology, College of Natural Sciences, Hanyang University) ;
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Kim, Kil-Soo
(Department of Laboratory Animal Research, Asan Institute for Life Science)
- 발행 : 1999.03.30
초록
당뇨병은 생식내분비 조직의 구조나 기능 변화를 유발하여 호르몬 합성 및 분비량의 변화를 야기하고, 정자의 운동성 등에 영향을 미치는 것으로 알려져 왔다. 그러나 부정소와 수정관내 정자의 농도 변화나 수정능력 획득 및 침체반응에 미치는 영향은 잘 알려져 있지 않다. 본 실험에서는 Wistar 쥐에 streptozotocin을 투여하여 당뇨병을 유발시킨 후 3일과 14일에 부정소 각 부위와 수정관내 정자 농도 변화를 조사하고 부정소 꼬리와 수정관내 정자의 침체반응 유도 실험 (acrosome reaction to ionophore challenge test, ARIC test)을 이용하여 정자의 수정 능력을 평가하였다. Streptozotocin을 주사한 후 혈액내 인슐린 및 포도당 농도는 당뇨병 경과 기간이 길어짐에 따라 반비례 관계를 보였다. 부정소 머리와 몸통내 정자의 농도는 3일군에서부터 감소하기 시작하나 꼬리에서는 14일군
Some of the information concerning sexual function in the male diabetes has been focused upon the problems of endocrine or semen parameters. However, the characteristics of acrosome reaction and spermatozoa concentration at the epididymis and vas deferens have scarcely been studied, and the causes of the infertility has not been critically identified. So, we designed to inspect the spermatozoa concentration and the characteristics of acrosome reaction at epididymis and vas deferens of diabetic Wistar rat induced by streptozotocin (STZ, 70 mg/kg, ip). Experimental animal was sacrificed at 3 days and 14 days after the STZ injection. In the diabetes-induced rat, the levels of insulin and glucose had a pattern of inverse proportion. The spermatozoa concentrations in caput and corpus epididymis were significantly decreased in all diabetic condition. In cauda epididymis, however, there was significant decrease in sperm concentration at 14 days onward. In diabetic rat, the spontaneous reaction rate of spermatozoa of cauda and vas deferens were significantly higher than the control group. The ARIC (acrosome reaction to ionophore challenge) value of caudal sperm was 28.7 at control, 22.1 at 3 days, and 8.3 at 14 days. In the present study the spermatozoa concentration was decreased and the spontaneous reaction rate was increased by diabetes. In ARIC-test, it is revealed that the fertility of spermatozoa of 14 days group was lower than control or 3 days group. Diabetes mellitus may be provoke the decreased fertilization rate and subsequent infertility and subsequent infertility.