KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)
- Volume 44 Issue 4
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- Pages.339-344
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- 1999
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- 0252-9777(pISSN)
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- 2287-8432(eISSN)
Development and Characterization of Anti-gliadin Polyclonal Antibody in Wheat
- Chang, Suk Joo (Deparment of Agronomy, Korea University) ;
- Hong, Byung Hee (Deparment of Agronomy, Korea University) ;
- Seo, Yang Weon (Deparment of Agronomy, Korea University)
- Published : 1999.12.01
Abstract
Immunological method has been applied in biochemical genetic analysis of seed storage proteins. We developed and characterized anti-gliadin polyclonal antibody (AGPab) specific to gliadin fractions whose quality and quantity were known to be associated with wheat end-use quality. Reactions of anti-gliadin polyclonal antibody (AGPab) to gliadin were linearly decreased as AGPab and antigen were diluted. Dot-blot and immunoblot assay showed that produced AGPab specifically reacted to gliadin and mainly