Cytotoxic Effect and Fatty Acid Composition of Lipopolysaccharide from Vibrio vulnificus

Vibrio vulnificus Lipopolysaccharide의 세포 독성 효과와 지방산 조성

Lipopolysaccharide(LPS) from Vibrio vulnificus was purified, the fatty acid composition was analyzed, and Limulus gelation activity and lethal toxic activity were tested in order to investigate the cause of cytotoxicity by V. vutnificus. These results were compared to those of Escherichia coli LPS and Salmonella typhimurium LPS. LPS from V. vulnificus had a different fatty acid composition from those of E coli and S. typhimurium. The major fatty arid from each LPS was lauric acid for E. coli, rapric acid for S. typhimurium, and myristic acid for V. vulnificus. The Limulus gelation activities of three LPSs were the same(0.1ng/ml) and the lethal toxicity in BALB/c mouse of V vulnificus LPS was similar to those of E. coli LPS and S. typhimurium LPS. Such factor as exotoxin need to be considered to be the cause of cytotoxicity by V. vulnificus LPS.

감염성 Vibrio vulnificus에 의한 패혈증과 같은 질병의 원인을 알아보기 위하여 Vibrio vulnificus로 부터 세포벽 lipopolysaccharide를 추출한 후 지방산 조성을 분석하고 limulus gelation activity와 lethal toxicity를 측정하였다. 이 결과들을 비감염성 Escherichia coli LPS와 감염성 Salmonella typhimurium LPS의 것들과 비교하였다. V. vulnificus LPS의 주 지방산은 myristic acid 이었고 E. coli LPS는 lauric acid, S. typhimurium LPS는 capric acid 이었다. 세가지 LPS의 Limulus gelation activity는 같았으며(0.1ng/ml), V. vulnificus LPS의 lethal toxicity는 E. coli LPS와 S. typhimurium LPS의 것과 비슷하였다. LPS 이외에도 exotoxin과 같은 인자들도 V. vulnificus에 의한 세포 독성의 원인으로 고려해야 할 것이다