Exo-inulinase 생산 균주의 분리ㆍ동정 및 효소 생산의 최적화

Isolation and identification of Exo-Inulinase Producing Bacterium and Optimization of the Enzyme Production

  • 발행 : 1999.02.01

초록

A bacterium producing exo-inulinase was isolated from soil and identified Pseudomonas sp. and named as Pseudomonas sp. NO5. The optimal culture conditions for the efficient production of exo-inulinase from Pseudomonas sp. NO5 were obtained by cultivating with the medium 1$\%$ sucrose, 0.5$\%$ yeast extract, 0.5$\%$ $(NH_4)_2$$HPO_4$, 0.05$\%$ $MgSO_4$$7H_2$0, 0.001$\%$ and $FeSO_4$$7H_2$0 at $37^{\circ}C$ in initial pH 7.0 for 20 hours. The enzyme was induced maximally in the presence of sucrose or inulin at early stationary phase about 20 hour after cultivation.

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