Immunohistochemical diagnosis of infectious pancreatic necrosis

어류 전염성훼장괴사증의 면역조직화학적 진단

  • Kim, Soon-Bok (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • 김순복 (경상대학교 수의과대학 동물의학연구소)
  • Published : 1999.06.01

Abstract

This experiment was carried out to establish the immunohistochemical diagnostic method for infectious pancreatic necrosis in the monolayers of CHSE-214 cell cultures and paraffin-embedded tissue sections from rainbow trout infected with infectious pancreatic necrosis virus(IPNV). Specific identification of IPNV antigens was often demonstrated in the pancreatic exocrine cells, and less in the intestinal mucous epithelia and the renal hemopoietic tissues by the use of monoclonal antibodies against capsid protein VP2. The specific reaction was seen as a distinct red cytoplasmic color, often as small granules of various sizes. The result showed that streptavidin alkaline phosphatase immunohistochemisry specifically identified IPNV antigens in both infected cell cultures and tissue sections.

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