Characterization of an Extracellular Cellulose-Hydrolyzing Enzyme Complex from a Thermotolerant Strain of Aspergillus sp.

  • Lusta, Konstantin A. (Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino Biological Center) ;
  • Chung, Il-Kyung (Faculty of Life Resources, Catholic University of Taegu-Hyosung) ;
  • Sul, Ill-Whan (Institute of Agricultural Technology and Science, Kyungpook National University) ;
  • Park, Hee-Sung (Faculty of Life Resources, Catholic University of Taegu-Hyosung) ;
  • Shin, Dong-Ill (Faculty of Life Resources, Catholic University of Taegu-Hyosung)
  • Published : 1999.12.01

Abstract

Aspergillus sp. CX-l strain grown on microcrystalline cellulose resulted in the accumulation of high levels of cellulase and xylanase activities that were higher by two to four folds than those from the conventional commercial producer, Trichoderma reesei QM9414. Aspergillus sp. CX-1 demonstrated greater thermo stability and better catalytic characteristics of total cellulase activity (FPase) as compared to T. reesei and Aspergillus niger F-2039.

Keywords

References

  1. Appl. Microbiol. Biotechnol. v.24 Enhaced cellulase production from Trichoderma reesei QM9414 on Physocally treated wheat straw Acebal, C.;M. P. Castillon;P. Estrada;I. Matal;E. Costa;J. Aguado;D. Romero;F. Jimenez
  2. FEMS Microbiol. Rev. v.13 The biological degradation of cellulose Beguin, P;J. P. Aubert
  3. Biotechnol. Bioeng. v.26 Effect of dilution on carboxymethylcellulse and xylanase assay Cauchon, N.;A. Le Duy
  4. Appl. Microbiol. Biotechnol. v.39 Production of celluase using a mutant strain of Trichoderma reesei Chaudhuri B. K.;V. Sahai
  5. Hemicellulose and Hemicellulases Coughlan, M. P.;G. P. Hazelwood
  6. J. Microbiol. Biotechnol. v.8 Isolation of microorganisms for biotechnological application Pranco, C. N. N.;N. C. McClure
  7. Pure Appl. Chem. v.59 Measurement of cellulase activities Ghose, T. K.
  8. App. Envion. Microbiol. v.63 Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei Ilmen, M.;A. Saloheimo ;M. L. Onnela;M. E. Penttila
  9. Process Biochem. v.26 Fungal celluloytic enzyme production Ingrid, P.;T. Folke;H. Barbel
  10. Pure Appl. Chem. v.59 International Union of Pure and Applied Chemistry
  11. Methods Enzymol. v.160 Xylanases and β-Xylosidase of Trichoderma lignorum John, M.;J. Schmidt
  12. Biochem. Soc. Trans. v.13 Applications of cellulases Mandels, M.
  13. Biotechnol. Bioeng. Symp. v.6 Measurement of saccharifying cellulase Mandels, M.;R. Andreotty;C. Roche
  14. Appl. Microbiol. Biotechnol. v.48 Genetic immobilization of cellulase on the cell surface of Saccharamyces cerevisiae Murai, T.;M. Ueda;H. Atomi;Y. Shibasaki;N. Kamasawa;M. Osumi;T. Kawaguchi;M. Arai;A. Tanaka
  15. Appl. Microbiol. Biotechnol. v.22 A comparison between the cellulase systems of Trichoderma harzianum E58 and Trichoderma reesei C30 Saddler, J. N.;C. M. Hogan;G. Lousi-Seize
  16. Eur. J. Biochem. v.249 cDNA cloning of Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast Saloheimo, M.;T. Nakari-Setaelae;M. Tenkanen;M. Penttilae
  17. Appl. Microbiol. Biotechnol. v.32 Xylanase production by Trichoderma harzianum E58 Senior, D. J.;P. R. Mayers;J. N. Saddler
  18. J. Microbiol. Biotechnol. v.8 Identification of multiple active forms in cellulase-xylanase of Aspergillus sp. 8-17 by active staining Shin, P. G.;J. B. Ahn;C. Y. Kim;W. H. Jeong;J. C. Ryu
  19. Appl. Environ. Microbiol. v.64 The transcriptional activator xInR regulates both xylanolytic and endoglucanase gene expression in Aspergillus niger Van Peij, N. M. E.;M. M. C. Gielkens;R. P. de Vries;J. Visser;L. H. de Graaff
  20. Microbiol. Rev. v.52 Multiplicity of β-1,4-xylanase in microorganisms: Functions and applications Wong, K. K. Y.;L. U. L. Tan;J. N. Saddler
  21. Methods Enzymol. v.160 Methods for measuring cellulase activities Wood, T. M.;K. M. Bhat
  22. J. Microbiol. v.34 Purification of carboxymethyl cellulase from hybrid between Aspergillus niger and Penicillum verruculosum Yang, Y. K.;J. S. Lee;H. N. Park;M. N. Moon;H. S. Kim;J. S. Kim;C. Y. Lim;Y. H. Rhee
  23. J. Microbiol. Biotechnol. v.8 Production of cellulase Trichoderma reesei Rut C30 in batch fermenter Yu, X. B.;H. S. Yun;Y. M. Koo
  24. Methods Enzymol. v.160 Methods for measuring cellulase activities Wood,T.M.;K.M.Bhat