Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Comparative Study of Immune-Enhancing Activity of Crude and Mannoprotein-Free Yeast-Gluean Preparations

  • Kim, Hye-Nam (Graduate School of Biotechnology, Korea University) ;
  • Lee, Jung-Nam (Graduate School of Biotechnology, Korea University) ;
  • Kim, Gi-Eun (Department of Biotechnology, Seokyeong University) ;
  • Ha-Lee, Young-Mie (Korea Nutrition Research Center, Korea University) ;
  • Kim, Chan-Wha (Graduate School of Biotechnology, Korea University) ;
  • Sohn, Jeong-Won (Graduate School of Biotechnology, Korea University, Department of Biochemistry, Korea University College of Medicine)
  • Published : 1999.12.01
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Abstract

${\beta}-Glucan$, one of the major cell wall components of Saccharomyces cerevisiae, is known to enhance the immune function, especially by activating macrophages. Accordingly, in an effort to develop a safe and efficient immune stimulatory agent, we prepared crude ${\beta}-glucan$ (glucan-p1) and partially purified ${\beta}-glucan$ that was free of mannoproteins (glucan-p2), and evaluated their effect on both the macrophage function and resistance to E. coli-induced peritonitis. To investigate the function of the macrophages, phagocytosis, $TNF-{\alpha}$ secretion, oxygen burst, and the expression of cytokine genes such as $IFN-{\gamma}$ and IL-12 were analyzed. Glucan-p2 markedly stimulated the macrophages with all these parameters. Glucan-p1, however, did not stimulate phagocytosis, yet it induced $TNF-{\alpha}$ secretion, oxygen burst, and the expression of $IFN-{\gamma}$ and IL-12, although less efficiently than glucan-p2. Finally, to test the in vivo protective effect of {\beta}-glucan against infection, the survival of mice from E. coli-induced peritonitis was investigated. After 24 h of the peritoneal challenge of E. coli, all of the mice treated with glucan-p2 survived whereas none survived in the control group. Glucan-p1 showed only a marginal effect in protecting the mice. These results suggest that mannoprotein-free gluean-p2, but not gluean-p1, can serve as an effective immune-stimulating agent.

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