Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genetic Analysis of Alcohol Yeasts Isolated from Korean Traditional Liquor by Polymerase Chain Reaction

  • Park, Heui-Dong (Department of Food Science and Technology, Kyungpook National University) ;
  • Kim, Seung-Hwan (Department of Food Science and Technology, Kyungpook National University) ;
  • Shin, Jae-Ho (Department of Agricultural Chemistry, Kyungpook National University) ;
  • Rhee, In-Koo (Department of Agricultural Chemistry, Kyungpook National University)
  • Published : 1999.12.01
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Abstract

Forty alcohol yeast strains were isolated from the main mashes (10 strains from each mash) for brewing of 4 different kinds of Korean traditional liquor (3 different types of Yakju and 1 Takju). Thirty-eight out of 40 strains were identified to be the same strain, Saccharomyces boulardii, by the Automated Bacteria, Yeast, and Fungi Identification System (Biolog Co., U.S.A.) based on the metabolic fingerprints. One strain that showed the highest ethanol production among the 38 strains in YPD medium, designated SHY 111, was selected and used for differentiating from other yeast type strains using the polymerase chain reaction (PCR). Amplified DNA, from transcribed internal spacers of SHY 111 chromosomal DNA, was found to be the same in both size and sequence as those of S. cerevisiae KCCM 11215 (formerly S. coreanus) and S. boulardii along with that of S. cerevisiae AB 972, which was used as a type strain for the yeast genome project. However, when PCR was carried out with the intron splice site primer, it resulted in the amplification of the SHY 111-specific DNA fragment which was about 200 bp in size. When PCR was carried out using the primer to test diversity of 40 isolated yeast strains, it was found that the PCR patterns were similar to each other except for the 200 bp bands derived from all the 10 strains from one Yakju, and 2 strains from another Yakju. These results suggest the strain identified as S. boulardii by the Automated Identification System to be a dominant strain for the fermentation of Korean traditional liquors.

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References

  1. Evolution v.43 Variation in ribosomal DNA among biological species of Armillaria, a genus of root-infecting fungi Anderson, J. B.;S. S. Bailey;P. J. Pukkila
  2. Mycologia v.84 Molecular phylogeny of northern hemispherer species of Armillaria Anderson, J. B.;E. S. Stasovski
  3. Yeast, Characteristics and Identification Barnett, J. A.;R. W. Payne;D. Yarrow
  4. Am. J. Enol. Vitic. v.40 Wine yeast strain identification Degre, D.;D. Y. Thomas;J. Ash;K. Mailhiot;A. Morin;C. Dubord
  5. Appl. Environ. Microbiol. v.62 PCR differentiation of commercial yeast strains using intron splice site primers de Miguel, B. L.;A. Soden;P. A. Henschke;P. Langridge
  6. Appl. Environ. Microbiol. v.50 Growth of natural yeast flora during the fermentation of inoculated wine Heard, G. M.;G. H. Fleet
  7. Foods Biotechnol. v.6 Microflora and physicochemical characteristics of Nuruk and main mashes during fermentation of a traditional Andong Soju Hong, Y.;Y. B. Kim;S. O. Park;E. H. Choi
  8. Nature v.387 The nucleotide sequence of Saccharomyces cerevisiae chromosome XII. Johnson, M.
  9. Int. J. Syst. Bacteriol. v.36 Electrophoretic karyotyping of laboratory and commercial strains of Saccharomyces and other yeasts Johnson, J. R.;R. K. Mortimer
  10. Methods in Yeast Genetics Kaiser, C.;S. Michaelis;A. Mitchell
  11. J. Kor. Agr. Chem. Soc. v.10 Microbiological and enzymological studies on Takju brewing Kim, C. J.
  12. Appl. Bkiochem. Biotechnol. v.44 Cloning of a new allelic variant of a Saccharomyces diastaticus glucoamylase gene and its introduction into industrial yeasts Kim, K.;G. Bazszar;S. Y. Lee;F. Knudsen;J. R. Mattoon
  13. J. Microbiol. Biotechnol. v.8 Strain improvement of yeast for ethanol production using a combined treatment of electric field and chemical mutagen N-methyl-N-nitro-N-nitrosoguanidine Kim, K.;J. Y. Lee
  14. Am. J. Enol. Vitic. v.45 PCR and DNA fingerprinting used as quality control in the production of wine yeast strains Lavallee, F.;Y. Salvas;S. Lamy;D. Y. Thomas;R. Degre;L. Dulau
  15. Kor. J. Microbiol. v.8 Studies on the microflora of Takju brewing Lee, J. S.;T. W. Rhee
  16. The Yeast, A Taxonomic Study Lodder, J.
  17. J. Microbiol. Biotechnol. v.8 Development of cellobiose-utilizing recombinant yeast for ethanol production from cellulose hydrolyzate Pack, S. P.;K. M. Cho;H. S. Kang;Y. J. Yoo
  18. Kor. J. Appl. Microbiol. Biotechnol. v.23 Identification of filamentous molds isolated from Korean traditional Nuruk and their amylolytic activities Park, J. W.;K. H. Lee;C. Y. Lee
  19. Methods Enzymol. v.194 DNA of Saccharomyces cerevisiae Philippsen, P.;A. Stotz;C. Scherf
  20. Appl. Environ. Microbiol. v.58 Molecular monitoring of wine fermentation conducted by active dry yeast strains Querol, A.;E. Barrio;T. Huerta;D. Ramon
  21. Syst. Appl. Microbiol. v.15 A comparative study of different methods of yeast strain characterization Qero, A.;E. Barrio;D. Ramon
  22. Am. J. Enol. Vitic. v.46 Use of random amplified polymorphic DNA (RAPD-PCR) in the characterization of wine yeasts Quesada, M. P.;J. L. Cenis
  23. Vitis v.7 The importance of yeasts in determining the composition and quality of wines Rankine, B. C.
  24. The Yeasts, A Taxonomic Study Rij, K.
  25. Zentralbl. Bakteriol. Parasitenk. v.26 Notizen ber einige Koreanische Garungsorganismen Saito, K.
  26. Molecular Cloning, A Laboratory Manual(2nd ed.) Sambrook, J.;E. F. Fritsch;T. Maniatis
  27. J. Appl. Bacteriol. v.75 Analysis of yeast diversity during spontaneous and induced alcoholic fermentation Schutz, M.;J. Gafner
  28. J. Microbiol. Biotechnol. v.4 Screening and characterization of thermotolerant alcohol-producing yeast Sohn, H. Y.;S. H. Seu
  29. Am. J. Bot. v.80 Molecular evolution and phylogenetic implications of internal transcribed spacer sequences of ribosomal DNA in witeraceae Suh, Y.;L. B. Thien;H. E. Reeve;E. A. Zimmer
  30. Kor. J. Food. Sci. v.28 Shelf-life and microbiological study of Sansung Takju Yang, J. Y.;K. H. Lee