Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Effect of Gluconic Acid on the Production of Cellulose in Acetobacter xylinum BRC5

  • PARK, SANG TAE (Molecular Microbiology Laboratory, Department of Biology, and Bioproducts Research Center, Yonsei University) ;
  • TAEKSUN SONG (Molecular Nmicrobiology Laboratory, Department of Biology, and Bioproducts Research Center, Yonsei University) ;
  • YOUNG MIN KIM (Molecular Nmicrobiology Laboratory, Department of Biology, and Bioproducts Research Center, Yonsei University)
  • Published : 1999.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Four mutants of Acetobacter xylinum BRC5 defective in gluconic acid production were isolated from UV-irradiated cells. The gluconic acid-negative mutants did not show glucose oxidase activity. The mutants were also defective in cellulose production. A randomly selected mutant grown in the Hestrin-Schramm medium (pH 6.0) supplemented with gluconic acid, however, was found to synthesize cellulose. The mutant grown in Hestrin-Schramm medium whose pH was adjusted to 5.0 with HC1 and contained no gluconic acid also produced cellulose. Wild-type cells grown under the same condition synthesized cellulose more rapidly than those grown in the pH 6.0 medium.

Keywords

References

  1. The Polysaccharide v.1;2;3 Aspinall, G. O.
  2. J. Bacteriol. v.84 Synthesis of cellulose from pyruvate by succinate-grown cells of Acetobacter xylinum Benziman, M.;H. Burger-Rachamimov
  3. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M.
  4. J. Bacteriol. v.157 Calcofluor staining of cellulose during microcyst differentiation in wild type and mutant strains of Polysphondylium pallodum Choi, A. H. C.;D. H. O'day
  5. Eur. J. Biochem. v.123 Biosynthesis of polysaccharides in Acetobacter xylinum: Sequential synthesis of a heptasaccharide diphosphate prenol Couso, R.;L. Ielpi;R. C. Garcia;M. A. Dankert
  6. Intl. J. Biol. Macromol. v.16 Genetic analysis of the acetan biosynthetiv pathway in Acetobacter xylinum Griffin, A. M.;V. J. Morris;M. J. Gasson
  7. Appl. Microbiol. v.16 Use of a fluorescent brightner to demonstrate cellulose in the cellular slime molds Harrington, B. J.;K. B. Raper
  8. Biochem. J. v.58 Synthesis of cellulose by Acetobacter xylinum. 2. Preparation of freeze-dried cells capable of polymerizing glucose to cellulose Hestrin, S.;M. Schramm
  9. Microbiol. Rev. v.50 Acetone-butanol fermentation revisited Jones, D. T.;D. R. Wood
  10. J. Ferment. Bioeng. v.83 Effect of agitator configuration on bacterial cellulose productivity in aerated and agitated culture Kouda, T.;H. Yano;F. Yoshinaga
  11. Biotechnol. Adv. v.8 Microbial cellulose as a speciality chemical Legge, R. L.
  12. J. Ferment. Bioeng. v.75 Production of cellulose from glucose by Acetobacter xylinum Masaoka, S.;T. Ohe;N. Sakota
  13. Experiments in Molecular Genetics(2nd ed.) Miller, J. H.
  14. Biosci. Biotechnol. Biochem. v.59 Production of cellulose from D-mannitol by Acetobacter xylinum KU-1 Oikawa, T.;T. Ohtori;M. Ameyama
  15. Microbiol. Rev. v.55 Cellulose biosynthesis and function in bacteria Ross, P.;R. Mayer;M. Benziman
  16. Nature v.179 Role of hexose phosphate in synthesis of cellulose by Acetobacter xylinum Schramm, M.;Z. Gromet;S. Hertin
  17. J. Ferment. Bioeng. v.85 Cellulose production by Acetobacter xylinum BRC5 under agitated condition Yang, Y. K.;S. H. Park;J. W. Hwang;Y. R. Pyun;Y. S. Kim