Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of a High-Yield Mutant Strain for L-Proline Production and Its Fermentation Conditions

  • Ryu, Wuk-Sang (Biochemical Process Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jang, Hyung-Wook (Biochemical Process Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Kyoung-Hee (Biochemical Process Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chang, Soon-Jae (Yang Ji Chemical Co., Ltd.) ;
  • Ryu, Yeon-Woo (Department of Molecular Science and Technology, Ajou University) ;
  • Park, Young-Hoon (Biochemical Process Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 1999.10.01
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Abstract

L-Proline-producing mutant strains were developed by exposing L-glutamic acid-producing bacteria to N-metyl-N-nitro-nitrosoguanidine and UV irradiation. A L-histidine auxotroph of Corynebacterium acetoacidophilum RYU3161(KCTC 0616BP), which was resistant to sulfaguanidine and proline analogs (DHP, AZC, TAC), was isolated. The activity of the mutant strain's $\gamma$-glutamyl kinase was 45% higher than that of the parent strain. The optimum level of L-histidine for production of L-proline was 0.16 g/l. In a 5-1 jar fermenter, the mutant strain produced L-proline at a high concentration (35 g/l) level within 48 h of cultivation.

Keywords

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