Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cultivation of Digitalis lanata Cell Suspension in an Aqueous Two-Phase System

  • Choi, Yeon-Sook (Department of Biological Engineering, Inha University) ;
  • Lee, Sang-Yoon (Department of Biological Engineering, Inha University) ;
  • Kim, Dong-Il (Department of Biological Engineering, Inha University)
  • Published : 1999.10.01
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Abstract

Suspension cultures of Digitalis lanata were successfully performed in an aqueous two-phase system (ATPS) of 4.5％ polyethylene glycol (PEG) 20,000 and 2.8％ crude dextran. Cell growth in the medium containing an individual ATPS-forming polymer was inhibited due to the toxicity of PEG and a high viscosity of dextran. Formation of ATPS supported cell growth by showing a considerably decrease in viscosity and partitioning of cells into a PEG-lean dextran phase. It was found that an aqueous two-phase cultivation of plant cells in a stirred tank bioreactor could be successfully applied.

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References

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