Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of a Novel Carbohydrase from Lipomyces starkeyi KSM 22 for Dental Application

  • KIM, DOMAN (Department of Biochemical Engineering, Research Institute for Catalysis, Chonnam National University, Research Center for New-Biomaterials in Agriculture, Seoul National University) ;
  • SU-JIN RYU (Department of Chemical Engineering, Chonnam National University) ;
  • SOO-JIN HEO (Department of Chemical Engineering, Chonnam National University) ;
  • DO-WON KIM (Department of Physics, Kangnung National University) ;
  • HO-SANG KIM (Research Center for New-Biomaterials in Agriculture, Seoul National University)
  • Published : 1999.06.01
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Abstract

The combined activities of dextranase and amylase(DXAMase) from Lipomyces starkeyi KSM 22 produced from starch fermentation inhibited or prevented dental plaque formation. The activities were stable in commercial mouthwash products; DXAMase activity retained over 93％ of original activity after 6 months at 23℃. We examined the effects of enzyme inhibitors and active ingredients in mouthwash on DXAMase activity. The DXAMase was stable with 0.29％(w/v) EDTA, 20％ (v/v) ethanol, 0.05％ (w/v) fluoride, and 0.05％ (w/v) SDS. Among the active ingredients of mouthwash, sodium benzoate (up to 1 ％, w/v) had no inhibitory effect on either dextranase or amylase activity. In the case of cetylpyridinium chloride, the addition of 0.05％ (w/v) inhibited 6％ of dextranase activity and 13％ of amylase activity. Propylene glycol (up to 1％, w/v) showed no inhibitory effect on either enzyme activity. DXAMase (5 IU/㎖) in mouthwash could remove pre-formed films of glucan-bound S. mutans cells. The addition of 0.1 IU/㎖ DXAMase in mouthwash prevented the formation of insoluble-glucan. These in vitro properties of L. starkeyi KSM 22 DXAMase are desirable for its application as a dental plaque control agent.

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