Journal of Microbiology and Biotechnology
- Volume 9 Issue 2
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- Pages.173-178
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- 1999
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Purification and Properties of Extracellular Cytosine Deaminase from Chromobacterium violaceum YK 391
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Yu, Tae-Shick
(Department of Microbiology, College of Natural Science, Keimyung University) ;
- Kim, Tae-Hyun (Department of Herbs and Food Science, Traditional Food Institute, Kyongbuk College of Science)
- Published : 1999.04.01
Abstract
The extracellular cytosine deaminase (EC 3.5.4.1) from Chromobacterium violaceum YK 391 was purified 264.7-fold with an overall yield of 14.3%. The enzyme was for the first time homogeneous by the criteria of polyacrylamide gel electrophoresis performed in the absence and in the presence of sodium dodecyl sulfate. The molecular weight of the purified enzyme was estimated to be about 156 kDa. The enzyme consisted of two identical subunits of approximate molecular weight 78 kDa. The isoelectric point of the enzyme was pH 5.55. The enzyme had a pH optimum of 7.5 and a temperature optimum of around 40 to