Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Involvement of Cytochrome P450 in (-)-(4R)-Isopiperitenone Oxidation by Cell Suspension Cultures of Mentha piperita

  • Park, Si-Hyung (Division of Applied Biology and Chemistry, and the Research Center for new Bio-Materials in Agriculture, Seoul National University) ;
  • Chang, Yung-Jin (Division of Applied Biology and Chemistry, and the Research Center for new Bio-Materials in Agriculture, Seoul National University) ;
  • Kim, Kyung-Hyun (Graduate School of Biotechnology, Korea University) ;
  • Kim, Soo-Un (Division of Applied Biology and Chemistry, and the Research Center for new Bio-Materials in Agriculture, Seoul National University)
  • Published : 1999.04.01
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Abstract

Biotransformation of exogenous (-)-(4R)-isopiperitenone in cell suspension cultures of Mentha piperita resulted in oxidized products, with (-)-7-hydroxyisopiperitenone being the major compound. The mass of products obtained $unde^{18}O_2$, atmosphere was two units higher than that under normal atmosphere. The biotransformation was inhibited by several cytochrome P450-specific inhibitors as well as by carbon monoxide. Carbon monooxide inhibition was substantially overcome by irradiation of cells with blue light including light at 450nm wavelength. These results suggested that a cytochrome P450-type monooxygenase was involved in the biotransformation.

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