Capsular Polysaccharide Serotypes Among Staphylococcus aureus Isolates from Cases of Bovine Mastitis and Dogs

젖소 유방염과 개에서 분리된 황색포도상구균에 대한 Capsular Polysaccharide형의 동정

  • Son-il Park (Department of Internal Medicine, Seoul National University)
  • Published : 1999.06.01

Abstract

본 연구는 임상형 및 준임상형 유방염에 이환된 젖소의 유즙에서 분리된 24주의 황색포도상구균에 대한 capsular polysaccharide(CP)형을 확인하고, 1% 토끼 혈청이 함유된 serum-soft agar 배지에서 집락의 모양을 관찰하였다. 또한 동물에 따라 우세한 CP형의 차이가 있는지를 비교하고자 개에서 분리된 13주에 대하여 동일한 실험을 수행하여 다음과 같은 결과를 얻었다. 집락모양을 관찰한 결과 젖소 유래 24주 중 16주(66.7%)는 diffuse, 5주(20.8%)는 compact, 나머지 3주(12.5%)는 분류가 불가능(indeterminate)하였다. CP형 확인결과 9주(37.5%)는 type 5, 2주(8.3%)는 type 8, 13주(54.2%)는 분류가 불가능(non-typeable)하였다. 한편, 개에서 분리된 균주 중 1주(type 8)를 제외한 12주(92.3%)는 type 5로 분류되었으며, 13주 중 8주(61.5%)가 diffuse형의 집락을 보였다. 본 실험에 사용한 균주의 수가 충분하지 못한 문제가 있지만 동물에 따라 우세한 CP형이 다를 수 있으며 분류 불가능한 균주가 상대적으로 높은 비율을 차지하였는데 이는 새로운 CP형의 분류형이 필요함을 시사하는 것으로 판단된다. 또한 type 5와 type 8만을 포함한 유방염 백신은 한계가 있을 것으로 사료된다.

Keywords

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