Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Responses of Transgenic Tobacco Plants Expressing Sweet Potato Peroxidases to Gamma Radiation

감마선에 대한 고구마 Peroxidase 형질전환 담배식물체의 반응

  • 윤병욱 (생명공학연구소 식물세포공학연구실) ;
  • 이행순 (생명공학연구소 식물세포공학연구실) ;
  • 권석윤 (생명공학연구소 식물세포공학연구실) ;
  • 김재성 (한국원자력연구소 동위원소ㆍ방사선응용연구팀) ;
  • 곽상수 (생명공학연구소 식물세포공학연구실)
  • Published : 1999.10.01
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Abstract

Transgenic tobacco plants expressing either a sweet potato anionic peroxidase (POD) (swpal) or neutral POD (swpnl) were irradiated by gamma radiation, and the gamma radiation-induced biochemical changes in antioxidant enzymes and plant growth inhibition were investigated at 30 days after treatment. Gamma radiation significantly inhibited the growth of all plants regardless of transgenic or nontransformed plants, showing a dose-dependent inhibition. In high dosage of 50 and 70 Gy, plant heights were severely retarded and new leaves does not emerged. No significant changes in antioxidant enzymes such as POD, superoxide dismutase and catalase were observed in all plants regardless of irradiation dosages ranging from 10 to 50 Gy. These results suggest that sweet potato PODs may be not involved in the protection against the oxidative stress induced by gamma radiation.

고구마 산성 peroxidase (POD) (swpal) 또는 중성 POD(swpnl)을 도입한 형질전환 담배에 다양한 선량의 감마선을 조사한 후 30일에 항산화효소 활성과 식물생장에 미치는 영향을 조사하였다. 감마선은 조사선량에 비례하여 형질전환 식물체와 정상식물에 관계없이 모든 식물체의 생장을 크게 억제시켰다. 50-70 Gy 처리에서 식물체의 신장이 크게 억제되었고 새로운 잎이 발생되지 못하였다. 10-50 Gy의 조사선량에서는 POD, superoxide dismutase, catalase 활성에 큰 변화를 주지 않았다. 이러한 결과는 고구마 POD는 감마선 조사에 의해 유도되는 산화적인 스트레스에 대한 보호효과에 관여하지 않음이 시사된다.

Keywords

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