Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Adventitious Bud Induction and Plant Regeneration from Mature Embryos in Larix leptolepis

낙엽송 (Larix leptolepis) 성숙배로부터 부정아 유도 및 식물체 재분화

  • Kim, Yong-Wook (Department of Tree breeding, Forestry Research Institute) ;
  • Kim, Joon-Chul (Department of Biology, Kangwon National University) ;
  • Youn, Yang (Department of Tree breeding, Forestry Research Institute) ;
  • Noh, Eu-Rae (Department of Tree breeding, Forestry Research Institute) ;
  • Son, Sung-Ho (Department of Tree breeding, Forestry Research Institute)
  • 김용욱 (임업연구원 임목육종부) ;
  • 김준철 (강원대학교 생물학과) ;
  • 윤양 (임업연구원 임목육종부) ;
  • 노의래 (임업연구원 임목육종부) ;
  • 손성호 (임업연구원 임목육종부)
  • Published : 1999.10.01
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Abstract

Adventitious buds were produced from the cultures of mature zygotic embryos of Larix leptolepis with the highest frequency of 91.7% in SH medium containing 1.0 mg/L zeatin. The most effective cytokinin combination for inducing adventitious buds was 1.0 mg/L zeatin + 1.0 mg/L thidiazuron (40.3%). The highest mean number of adventitious buds induced in 1.0 mg/L zeatin + 1.0 mg/L 2iP or 1.0 mg/L zeatin + 1.0 mg/L kinetin combined treatments. Elongation of the adventitious buds occurred the best on half strength LM salts medium, on which the buds elongated upto 27 mm. Also, the supplement of activated charcoal in medium suppressed the elongation of the adventitious buds. The highest frequency (23.3%) of rooting from elongated shoots was obtained on the medium containing 5.0 mg/L IBA and 0.2 mg/L NAA. The highest number (3.5) of roots was induced on the medium containing 5.0 mg/L IBA alone.

본 연구는 낙엽송 (Larix leptolepis)의 종자배로부터 배배양을 통한 식물체 재분화까지의 최적조건을 구명코저 수행되었는데 그 결과는 다음과 같이 요약할 수 있다. 최대 부정아 유도율은 SH배지에 1.0mg/L zeatin을 첨가한 처리구에서 가장 높은 91.7%의 부정아 유도율을 보였으며, cytokinin 혼용처리를 한 부정아 유도는 1.0mg/L zeatin + 1.0mg/L TDZ처리구에서 40.3%로 가장 높은 유도율을 보였다. 그리고 평균 부정아 유도수는 1.0mg/L zeatin+1.0 mg/L 2iP 및 1.0mg/L zeatin+1.0 mg/L kinetin 처리구에서 종자배당 1.9개로 동일한 유도수를 보여 그 효과는 비슷하였다. 부정아의 길이생장은 염류량을 절반으로 줄인 1/2LM배지에서 계대배양하였을 때 가장 높은 27mm의 신초가 생장되어 가장 효과적인 처리구 나타났다. 증식된 신초의 발근율은 5.0 mg/L IBA 및 0.2mg/L NAA 첨가시 23.3%로 가장 높았고 신초에서 유도된 뿌리수는 5.0mg/L IBA처리구에서 신초당 3.5개로 가장 많았다.

Keywords

References

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